Affinity of drugs for cytochrome P-450 determined by inhibition of p-nitrophenetole O-deethylation by rat liver microsomes.

Abstract

The rate of conversion of p-nitrophenetole to p-nitrophenol by rat liver microsomes was studied. Inhibition of the reaction by CO and by SKF 525A and the absolute dependence on NADPH and oxygen indicate that cytochrome P-450 catalyzes the reaction. The apparent Km for oxygen was 0.07 microM. Furthermore, cytochrome b5 seemed to be involved in the formation… (More)

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@article{Jrgensen1983AffinityOD, title={Affinity of drugs for cytochrome P-450 determined by inhibition of p-nitrophenetole O-deethylation by rat liver microsomes.}, author={L. B. J\orgensen and Terje Johansen}, journal={Acta pharmacologica et toxicologica}, year={1983}, volume={53 1}, pages={70-7} }