Affinity measurement of single chain antibodies: a mathematical method facilitated by statistical software SigmaPlot.


Because they are monovalent for antigen, single chain antibodies display a different antibody-antigen interaction pattern from that of full-length antibodies. Using the law of mass action and considering the antibody-antigen binding pattern at OD-100% and OD-50% points, we introduced a formula for estimating single chain antibody affinity. Sigmoid curves of… (More)
DOI: 10.1089/mab.2013.0067


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