Affinity maturation generates greatly improved xyloglucan-specific carbohydrate binding modules

@inproceedings{Schantz2009AffinityMG,
  title={Affinity maturation generates greatly improved xyloglucan-specific carbohydrate binding modules},
  author={Laura von Schantz and Fredrika Gullfot and Sebastian Scheer and Lada H. Filonova and Lavinia Cicortas Gunnarsson and James E. Flint and Geoffrey Daniel and Eva Nordberg-Karlsson and Harry Brumer and Mats Ohlin},
  booktitle={BMC biotechnology},
  year={2009}
}
BackgroundMolecular evolution of carbohydrate binding modules (CBM) is a new approach for the generation of glycan-specific molecular probes. To date, the possibility of performing affinity maturation on CBM has not been investigated. In this study we show that binding characteristics such as affinity can be improved for CBM generated from the CBM4-2 scaffold by using random mutagenesis in combination with phage display technology.ResultsTwo modified proteins with greatly improved affinity for… CONTINUE READING

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Novel xylanbinding properties of an engineered family 4 carbohydratebinding

L Cicortas Gunnarsson, C Montanier, +4 authors M Ohlin
  • 2007
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