Affinity labelling and characterization of the ppp(A2'p)nA-dependent endoribonuclease from different mammalian sources.

Abstract

The ppp(A2'p)nA-dependent endoribonucleases from a number of different mammalian sources have been investigated. The enzyme from reticulocyte lysates shows optimal activity of 50-150 mM KCl and requires the presence of Mg2+. Whilst the enzyme is inactivated after passage of reticulocyte lysates through Sephadex columns in the absence of ATP, it retains full… (More)

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@article{Wreschner1982AffinityLA, title={Affinity labelling and characterization of the ppp(A2'p)nA-dependent endoribonuclease from different mammalian sources.}, author={Daniel H. Wreschner and Robert Silverman and Tharappel C. James and Christopher S. Gilbert and Ian M. Kerr}, journal={European journal of biochemistry}, year={1982}, volume={124 2}, pages={261-8} }