Affinity labeling the ribosome with eukaryotic-specific antibiotics: (bromoacetyl)trichodermin.

@article{Gilly1985AffinityLT,
  title={Affinity labeling the ribosome with eukaryotic-specific antibiotics: (bromoacetyl)trichodermin.},
  author={M. Sue Gilly and Nicola Benson and Monica Pellegrini},
  journal={Biochemistry},
  year={1985},
  volume={24 21},
  pages={
          5787-92
        }
}
Trichodermin, a eukaryotic-specific antibiotic, inhibits protein synthesis in Drosophila cells. We have synthesized a 14C-labeled bromoacetyl derivative of trichodermin that binds to Drosophila 80S ribosomes and once bound reacts covalently with ribosomal proteins. It does not react with rRNA. Three large-subunit proteins (L1, L3, and L24) and three small-subunit proteins (S3/S5, 2/3S, and S8) are labeled by [14C] (bromoacetyl)trichodermin. Reaction with each of these proteins can be competed… CONTINUE READING

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