Affinity labeling of tubulin's exchangeable guanosine 5'-triphosphate binding site.

Abstract

Tubulin requires GTP for maximal rate and extent of polymerization into microtubules. The localization of the guanine nucleotide in the microtubule was examined by preparing affinity probes that would permit tubulin polymerization prior to their covalent coupling to amino acids in tubulin's exchangeable GTP binding site. Two different hydrolyzable GTP… (More)

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@article{Maccioni1983AffinityLO, title={Affinity labeling of tubulin's exchangeable guanosine 5'-triphosphate binding site.}, author={Ricardo Benjamin Maccioni and Nicholas W. Seeds}, journal={Biochemistry}, year={1983}, volume={22 7}, pages={1572-9} }