Affinity labeling of GTP-binding proteins in cellular extracts.

@article{Lw1992AffinityLO,
  title={Affinity labeling of GTP-binding proteins in cellular extracts.},
  author={Andreas L{\"o}w and Holger Faulhammer and Mathias Sprinzl},
  journal={FEBS letters},
  year={1992},
  volume={303 1},
  pages={
          64-8
        }
}
GTP-binding proteins in cellular extracts from Escherichia coli, Thermus thermophilus, yeast, wheat germ or calf thymus were identified using in situ periodate-oxidized [alpha-32P]GTP as affinity label. Site-specific reaction of individual GTP-binding proteins was achieved by cross-linking the protein-bound 2',3'-dialdehyde derivative of GTP with the single lysine residue of the conserved NKXD sequence through Schiff's base formation and subsequent cyanoborohydride reduction. Labeled GTP… CONTINUE READING
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