Affinity improvement of a therapeutic antibody to methamphetamine and amphetamine through structure-based antibody engineering

@inproceedings{Thakkar2014AffinityIO,
  title={Affinity improvement of a therapeutic antibody to methamphetamine and
amphetamine through structure-based antibody engineering},
  author={Shraddha Thakkar and Nisha Nanaware-Kharade and Reha Celikel and Eric C. Peterson and Kottayil I. Varughese},
  booktitle={Scientific reports},
  year={2014}
}
Methamphetamine (METH) abuse is a worldwide threat, without any FDA approved medications. Anti-METH IgGs and single chain fragments (scFvs) have shown efficacy in preclinical studies. Here we report affinity enhancement of an anti-METH scFv for METH and its active metabolite amphetamine (AMP), through the introduction of point mutations, rationally designed to optimize the shape and hydrophobicity of the antibody binding pocket. The binding affinity was measured using saturation binding… CONTINUE READING
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