Affinity chromatography on immobilized anhydrotrypsin: general utility for selective isolation of C-terminal peptides from protease digests of proteins.

  title={Affinity chromatography on immobilized anhydrotrypsin: general utility for selective isolation of C-terminal peptides from protease digests of proteins.},
  author={T. Kumazaki and K. Terasawa and S. Ishii},
  journal={Journal of biochemistry},
  volume={102 6},
Recently we have succeeded in the efficient isolation of the C-terminal peptides from tryptic digests of the tail sheath protein (with C-terminal Gly) and the tube protein (with C-terminal Glu) of bacteriophage T4, by taking advantage of a unique property of immobilized anhydrotrypsin, that is, a strong specific affinity for peptides containing Arg or Lys residues at their C-termini. In this study, the utility of affinity chromatography on immobilized anhydrotrypsin was further demonstrated in… Expand
Selective isolation of C-terminal peptides from proteolytic mixtures of proteins by affinity chromatography on immobilized anhydrotrypsin and anhydrochymotrypsin
Anhydrotrypsin in an immobilized form shows strong specific affinity for the peptides containing Arg, Lys, or S-aminoethyl-Cys at their C-termini. By taking advantage of this unique property of theExpand
Immobilized Anhydrotrypsin as a Specific Affinity Adsorbent for Polypeptides Containing Arginyl, Lysyl, or S-Aminoethylcysteinyl Residues at the C-termini
Reliable methods for sequence analysis from the C-terminus of a protein are still lacking unlike the case of that from the N-terminus. The most promising way for analysis of the C-terminal region, atExpand
Specific isolation by anhydrotrypsin‐agarose chromatography of a recombinant protein tagged with an affinity tail arginine at the C‐terminus
A characteristic property of anhydrotrypsin, i.e., its ability to strongly bind C‐terminal arginine, proved to be useful as a tool for specific enrichment of a recombinant protein. An arginine tailExpand
Application of an anhydrotrypsin-immobilized precolumn for selectvie separation of peptides having arginine or lysine at theinr C-termini by column-switching high-performance liquid
A column-switching high-performance liquid chromatographic (CS-HPLC) system which consisted of an anhydrotrypsin (AHT)-immobilized diol—silica precolumn and a reversed-phase analytical column wasExpand
Trypsin and affinity chromatography.
  • K. Kasai
  • Chemistry, Medicine
  • Journal of chromatography
  • 1992
Affinity adsorbents for trypsin which were prepared by immobilizing product-type ligands, that is, peptides having C-terminal arginine, proved to be effective not only for preparative purposes butExpand
Selective Isolation of C-Terminal Peptides by Affinity Chromatography
Every protein expresses its function through its capability of specific molecular recognition, which depends on its three-dimensional structure. In order to understand the fundamental relationExpand
Preparation of anhydrotrypsin-immobilized diol silica as a selective adsorbent for high-performance affinity chromatography of peptides containing arginine or lysine at their C-termini
SummaryAnhydrotrypsin (AHT), a catalytically inert derivative of trypsin in which the active site serine residue was converted to dehydroalanine residue by chemical modification, was immobilized ontoExpand
Preparation of anhydrochymotrypsin diol silica as selective adsorbent for affinity chromatography of peptides with aromatic amino acids at C-termini
SummaryAnhydrochymotrypsin (AHC), a catalytically inert derivative of chymotrypsin in which the serine-residue active site has been converted chemically to a dehydroalanine residue, was immobilizedExpand
Anhydroelastase: Enhanced affinity toward product‐type ligands revealed by affinity chromatography
Immobilized anhydroelastase was effectively isolated by a single operation of affinity chromatography from a complex mixture produced by phenylmethylsulfonylation and alkaline treatment of porcine pancreatic elastase and found to be useful in the purification and search for naturally occurring proteinase inhibitors. Expand
Improvement in Selectivity of Anhydrotrypsin-Immobilized Diol Silica Column by the Use of Eluent Containing Calcium Ion
Abstract The disadvantage of anhydrotrypsin-immobilized diol silica column, retention of basic peptides having no Arg or Lys at their C-termini and no retention of certain C-termina! Arg peptide, wasExpand