Affinity capturing and gene assignment of soluble glycoproteins produced by the nematode Caenorhabditis elegans.

@article{Hirabayashi2002AffinityCA,
  title={Affinity capturing and gene assignment of soluble glycoproteins produced by the nematode Caenorhabditis elegans.},
  author={Jun Hirabayashi and Ko Hayama and Hiroyuki Kaji and Toshiaki Isobe and Ken-ichi Kasai},
  journal={Journal of biochemistry},
  year={2002},
  volume={132 1},
  pages={103-14}
}
Protein glycosylation is a central issue for post-genomic (proteomic) sciences. We have taken a systematic approach for analyzing soluble glycoproteins produced in the nematode Caenorhabditis elegans. The approach aims at assigning (i) genes that encode glycoproteins, (ii) sites where glycosylation occurs, and (iii) types of attached glycan structures. A soluble extract of C. elegans, as a starting material, was applied first to a concanavalin A (ConA) column (specific for high-mannose type N… CONTINUE READING
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