Affinity capillary electrophoresis for the assessment of binding affinity of carbohydrate‐based cholera toxin inhibitors

  title={Affinity capillary electrophoresis for the assessment of binding affinity of carbohydrate‐based cholera toxin inhibitors},
  author={Oier Aizpurua‐Olaizola and Javier Sastre Tora{\~n}o and Aliaksei V. Pukin and Ou Fu and Geert‐Jan Boons and Gerhardus J. de Jong and Roland J. Pieters},
Developing tools for the study of protein carbohydrate interactions is an important goal in glycobiology. Cholera toxin inhibition is an interesting target in this context, as its inhibition may help to fight against cholera. For the study of novel ligands an affinity capillary electrophoresis (ACE) method was optimized and applied. The method uses unlabeled cholera toxin B‐subunit (CTB) and unlabeled carbohydrate ligands based on ganglioside GM1‐oligosaccharides (GM1os). In an optimized method… 
The assessment of Pseudomonas aeruginosa lectin LecA binding characteristics of divalent galactosides using multiple techniques
The affinity of a divalent ligand was determined to be in the low-nanomolar range for all of the used techniques and with a ligand residence time of approximately 7 h, while no strong binding was seen to related lectin tetramers.
Determination of binding constants for strong complexation by affinity capillary electrophoresis: the example of complexes of ester betulin derivatives with (2-hydroxypropyl)-γ-cyclodextrin
The features of using mobility shift affinity capillary electrophoresis for studying strong complexation and the permissible excess of analyte concentration over ligand concentration was found to be approximately 10–35, provided that the parameter a 1 was used, but the peak shape should be used as a landmark.
Mannose Receptors of Alveolar Macrophages as a Target for the Addressed Delivery of Medicines to the Lungs
An investigation of the structure, properties, and functions of the mannose receptors of the alveolar macrophages which were promising targets for the creation of systems of the addressed delivery of medicines for treatment of diseases of the upper airways is investigated.
Affinity Interactions by Capillary Electrophoresis: Binding, Separation, and Detection.
All molecules within and outside the cells, such as proteins, nucleic acids, hormones, and nutrients play vital roles through affinity interaction network in regulating fundamental life processes.
Synthesis of New Reagents as a ligands and Study of ( Investigation , Chromatographic Behavior , Solubility )
Formazane ligands are described by many chemical and physical properties that have increased their applications in several fields and therefore have been classified as reagents or ligands with donor
Binding affinity in drug design: experimental and computational techniques
This review discusses both experimental and computational approaches for affinity evaluation, and provides a guide to affinity predictions, collectively covering several techniques that are used in the first stages of rational drug design.
Nano crystalline cellulose sulfuric acid (s-NCC): a novel green nanocatalyst for the synthesis of polyhydroxy pyrimidine-fused heterocyclic compounds (PPFHs)
In this paper, rod-shaped nanocrystalline cellulose (NCC) was prepared from microcrystalline cellulose through mechanical method using ultrasonication. The resultant has been utilized to obtain


Screening method of carbohydrate-binding proteins in biological sources by capillary affinity electrophoresis and its application to determination of Tulipa gesneriana agglutinin in tulip bulbs.
Capillary affinity electrophoresis (CAE) will be a valuable method for both screening of lectins in natural sources and determination of their detailed carbohydrate-binding specificities.
Capillary affinity electrophoresis for the screening of post-translational modification of proteins with carbohydrates.
It is found that the lectins employed in the present study could discriminate subtle difference in linkages and resolved the carbohydrate mixtures, useful, for example, to understand the biological events expressed with carbohydrate changes on the cell surface.
Fighting Cholera One-on-One: The Development and Efficacy of Multivalent Cholera-Toxin-Binding Molecules.
  • H. Zuilhof
  • Chemistry, Biology
    Accounts of chemical research
  • 2016
A series of diseases, ranging from cholera via travelers' diarrhea to hamburger disease, are caused by bacterially produced toxic proteins. In particular, a toxic protein unit is brought into the
Affinity capillary electrophoresis for the determination of binding affinities for low molecular weight heparins and antithrombin‐III
ACE is used in this work to measure the relative AT‐III binding affinities of the low molecular weight heparins (LWMHs) dalteparin, enoxaparin, and tinzaparin and the synthetic pentasaccharide drug fondaparinux (Arixtra).
Picomolar inhibition of cholera toxin by a pentavalent ganglioside GM1os-calix[5]arene.
This work reports the first GM1os-based CT inhibitor that matches the valency of the CT binding domain (CTB), and contains five GM1OS moieties linked to a calix[5]arene scaffold that represents a significant multivalency effect, with a relative inhibitory potency of 100,000 compared to a monovalentGM1os derivative.
Characterization of Influenza Hemagglutinin Interactions with Receptor by NMR
The receptor binding properties of influenza A HA from different subtypes have been characterized by NMR spectroscopy and it is found that H5-Qinghai and H9-Hong Kong HA bind to both receptor analogs with similar affinity.
Notable Aspects of Glycan-Protein Interactions
This mini review highlights several interesting aspects of glycan-mediated interactions that are common between cells, bacteria, and viruses. Glycans are ubiquitously found on all living cells, and
Capillary electrophoresis of intact basic proteins using noncovalently triple-layer coated capillaries.
The feasibility of using the PB-DS-PB coated capillaries for CE with mass spectrometric detection was shown by the characterization of the impure llama antibody sample, revealing the presence of a side product in one of the antibodies.