Affinity capillary electrophoresis for the assessment of binding affinity of carbohydrate‐based cholera toxin inhibitors

@article{AizpuruaOlaizola2018AffinityCE,
  title={Affinity capillary electrophoresis for the assessment of binding affinity of carbohydrate‐based cholera toxin inhibitors},
  author={Oier Aizpurua‐Olaizola and Javier Sastre Tora{\~n}o and Aliaksei V. Pukin and Ou Fu and Geert‐Jan Boons and Gerhardus J. de Jong and Roland J. Pieters},
  journal={ELECTROPHORESIS},
  year={2018},
  volume={39}
}
Developing tools for the study of protein carbohydrate interactions is an important goal in glycobiology. Cholera toxin inhibition is an interesting target in this context, as its inhibition may help to fight against cholera. For the study of novel ligands an affinity capillary electrophoresis (ACE) method was optimized and applied. The method uses unlabeled cholera toxin B‐subunit (CTB) and unlabeled carbohydrate ligands based on ganglioside GM1‐oligosaccharides (GM1os). In an optimized method… 
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