Affinity capillary electrophoresis for the determination of binding affinities for low molecular weight heparins and antithrombin‐III

  title={Affinity capillary electrophoresis for the determination of binding affinities for low molecular weight heparins and antithrombin‐III},
  author={Meredith M Dinges and Kemal Solakyildirim and Cynthia K Larive},
The anticoagulant properties of heparin stem in part from high‐affinity binding to antithrombin‐III (AT‐III) inducing a 300‐fold increase in its inhibitory activity against the coagulation protease factor Xa. The minimal structural requirements for AT‐III binding are contained in the rare heparin pentasaccharide sequence containing a 3,6‐O‐sulfated N‐sulfoglucosamine residue. ACE is used in this work to measure the relative AT‐III binding affinities of the low molecular weight heparins (LWMHs… 

Using affinity capillary electrophoresis and computational models for binding studies of heparinoids with p‐selectin and other proteins

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