Affinity capillary electrophoresis for the determination of binding affinities for low molecular weight heparins and antithrombin‐III

  title={Affinity capillary electrophoresis for the determination of binding affinities for low molecular weight heparins and antithrombin‐III},
  author={Meredith M Dinges and Kemal Solakyildirim and Cynthia K Larive},
The anticoagulant properties of heparin stem in part from high‐affinity binding to antithrombin‐III (AT‐III) inducing a 300‐fold increase in its inhibitory activity against the coagulation protease factor Xa. The minimal structural requirements for AT‐III binding are contained in the rare heparin pentasaccharide sequence containing a 3,6‐O‐sulfated N‐sulfoglucosamine residue. ACE is used in this work to measure the relative AT‐III binding affinities of the low molecular weight heparins (LWMHs… 

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