Affinity-based isolation of tagged nuclei from Drosophila tissues for gene expression analysis.

Abstract

Drosophila melanogaster embryonic and larval tissues often contain a highly heterogeneous mixture of cell types, which can complicate the analysis of gene expression in these tissues. Thus, to analyze cell-specific gene expression profiles from Drosophila tissues, it may be necessary to isolate specific cell types with high purity and at sufficient yields… (More)
DOI: 10.3791/51418

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Cite this paper

@article{Ma2014AffinitybasedIO, title={Affinity-based isolation of tagged nuclei from Drosophila tissues for gene expression analysis.}, author={Jingqun Ma and Vikki M Weake}, journal={Journal of visualized experiments : JoVE}, year={2014}, volume={85} }