Affinity and rate constants for interactions of bovine folate-binding protein and folate derivatives determined by optical biosensor technology. Effect of stereoselectivity.

@article{NygrenBabol2005AffinityAR,
  title={Affinity and rate constants for interactions of bovine folate-binding protein and folate derivatives determined by optical biosensor technology. Effect of stereoselectivity.},
  author={Linn{\'e}a Nygren-Babol and Ase Sternesj{\"o} and Margareta J{\"a}gerstad and Lennart Bj{\"o}rck},
  journal={Journal of agricultural and food chemistry},
  year={2005},
  volume={53 13},
  pages={5473-8}
}
The interactions between bovine folate-binding protein (FBP) and different folate derivatives in pure diastereoisomeric forms were studied at pH 7.4 by a surface plasmon resonance technology (Biacore). The results show that folic acid had the most rapid association rate (k(a) = 1.0 x 10(6) M(-)(1) s(-)(1)), whereas (6S)-5-HCO-5,6,7,8-tetrahydrofolic acid had the most rapid dissociation rate (k(d) = 3.2 x l0(-)(3) s(-)(1)). The equilibrium dissociation constant (K(D)), calculated from the… CONTINUE READING