Affinity Purification of Mammalian RNA Polymerase I

  title={Affinity Purification of Mammalian RNA Polymerase I},
  author={R. Hannan and W. M. Hempel and A. Cavanaugh and T. Arino and S. Dimitrov and T. Moss and L. Rothblum},
  journal={The Journal of Biological Chemistry},
  pages={1257 - 1267}
  • R. Hannan, W. M. Hempel, +4 authors L. Rothblum
  • Published 1998
  • Biology, Medicine
  • The Journal of Biological Chemistry
  • Overlapping cDNA clones encoding the two largest subunits of rat RNA polymerase I, designated A194 and A127, were isolated from a Reuber hepatoma cDNA library. Analyses of the deduced amino acid sequences revealed that A194 and A127 are the homologues of yeast A190 and A135 and have homology to the β′ and β subunits of Escherichia coli RNA polymerase I. Antibodies raised against the recombinant A194 and A127 proteins recognized single proteins of approximately 190 and 120 kDa on Western blots… CONTINUE READING
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