Affinity—based screening of combinatorial libraries using automated, serial-column chromatography

Abstract

We have developed an automated serial chromatographic technique for screening a library of compounds based upon their relative affinity for a target molecule. A “target” column containing the immobilized target molecule is set in tandem with a reversed-phase column. A combinatorial peptide library is injected onto the target column. The target-bound… (More)
DOI: 10.1038/nbt0496-504

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