Affinity, potency and efficacy of tramadol and its metabolites at the cloned human µ-opioid receptor

@article{Gillen2000AffinityPA,
  title={Affinity, potency and efficacy of tramadol and its metabolites at the cloned human µ-opioid receptor},
  author={Clemens Gillen and Michael Haurand and D. Kobelt and Stephan Wnendt},
  journal={Naunyn-Schmiedeberg's Archives of Pharmacology},
  year={2000},
  volume={362},
  pages={116-121}
}
Abstract. The present study was conducted to characterise the centrally active analgesic drug tramadol hydrochloride [(1RS,2RS)-2-[(dimethyl-amino)-methyl]-1-(3-methoxyphenyl)-cyclohexanol hydrochloride] and its metabolites M1, M2, M3, M4 and M5 at the cloned human µ-opioid receptor. Membranes from stably transfected Chinese hamster ovary (CHO) cells were used to determine the four parameters of the ligand-receptor interaction: the affinity of (±)-tramadol and its metabolites was determined by… 
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