Aerosol transfer of bladder urothelial and smooth muscle cells onto demucosalized colonic segments: a pilot study.

@article{Hafez2003AerosolTO,
  title={Aerosol transfer of bladder urothelial and smooth muscle cells onto demucosalized colonic segments: a pilot study.},
  author={Ashraf T. Hafez and Darius J. Bagli and Andr{\'e} Bahoric and Karen Aitken and Charles Ray Smith and Daniel Herz and Antoine C. El Khoury},
  journal={The Journal of urology},
  year={2003},
  volume={169 6},
  pages={
          2316-9; discussion 2320
        }
}
PURPOSE We developed a cell transfer technology for covering demucosalized colonic segments with bladder urothelium. This covering would be achieved through aerosol spraying of single cell suspension of bladder urothelial and smooth muscle cells with fibrin glue onto the demucosalized colonic segments. MATERIALS AND METHODS In 6 piglets (20 kg.) a 4 cm.2 area of bladder was excised. Single cell suspension of bladder urothelial and smooth muscle cells was prepared. A segment of detubularized… CONTINUE READING
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