Aerobic production of alanine by Escherichia coli aceF ldhA mutants expressing the Bacillus sphaericus alaD gene


Alanine was produced from glucose in an Escherichia coli aceF ldhA double mutant strain that contained the pTrc99A-alaD plasmid expressing Bacillus sphaericus alanine dehydrogenase. The aceF gene encodes one of the proteins of the pyruvate dehydrogenase complex, and therefore this strain required acetate as an additional carbon source. The ldhA gene encodes… (More)
DOI: 10.1007/s00253-004-1560-3


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