Aerobic culture of methanogenic archaea without an external source of hydrogen

  title={Aerobic culture of methanogenic archaea without an external source of hydrogen},
  author={Saber Khelaifia and Jean-Christophe Lagier and Vanessa Demonfort Nkamga and Elodie Guilhot and Michel Drancourt and Didier Raoult},
  journal={European Journal of Clinical Microbiology \& Infectious Diseases},
Culturing methanogenic archaea is fastidious, expensive, and requires an external source of hydrogen and carbon dioxide. [] Key Method To obtain colonies, we cultivated M. smithii in an agar plate in the upper part of a double chamber flask with a liquid culture of B. thetaiotaomicron in the lower compartment. We subsequently cultured four other methanogenic species for the first time and successfully isolated 13 strains of M. smithii and nine strains of Methanobrevibacter oralis from 100 stools and 45 oral…
Isolation and culture of Methanobrevibacter smithii by co-culture with hydrogen-producing bacteria on agar plates.
Culture of salivary methanogens assisted by chemically produced hydrogen
An alternative method for hydrogen production using iron filings and acetic acid with the aim of cultivating methanogens more efficiently and more quickly is developed with a reference strain of Methanobrevibacter oralis.
Culture of previously uncultured members of the human gut microbiota by culturomics
It is shown that the use of culturomics allows the culture of organisms corresponding to sequences previously not assigned, which doubles the number of species isolated at least once from the human gut.
Culture of Methanogenic Archaea from Human Colostrum and Milk
It is suggested that breastfeeding may contribute to the vertical transmission of these microorganisms and may be essential to seed the infant’s microbiota with these neglected critical commensals from the first hour of life.
Detection of Methanobrevobacter smithii and Methanobrevibacter oralis in Lower Respiratory Tract Microbiota
Observations extend the known territory of methanogens to the respiratory tract and lay the foundations for further interpretation of their detection as pathogens in any future cases of isolation from bronchoalveolar lavages and the lungs.
Co-culture of Methanobrevibacter smithii with enterobacteria during urinary infection
Methanobrevibacter smithii, a methanogen consistently colonising the newborn stomach
A polyphasic approach, including microscopic observation by fluorescence in situ hybridisation, polymerase chain reaction (PCR) sequencing detection, identification and culture, is developed to isolate and genotype M. smithii in one-day-old newborns’ gastric juice specimens.
Methanogens as emerging pathogens in anaerobic abscesses
Clinical microbiology laboratories should work toward developing cheap and easy protocols for the routine detection and identification of methanogens in selected specimens in order to refine the diagnosis of infections, as well as to expand the knowledge about this group of intriguing microorganisms.


A Versatile Medium for Cultivating Methanogenic Archaea
Background Methanobrevibacter smithii, Methanobrevibacter oralis, Methanosphaera stadtmanae, Methanomassilicoccus luminyensis and Methanobrevibacter arboriphilicus have been cultured from human
Methanosphaera stadtmaniae gen. nov., sp. nov.: a species that forms methane by reducing methanol with hydrogen
The composition of its cell wall and 16 S rRNA and its immunological fingerprint are consistent with characterization of the organism as a member of a new genus of the family Methanobacteriaceae.
The roles of acetotrophic and hydrogenotrophic methanogens during anaerobic conversion of biomass to methane: a review
The aim of this paper is primarily to review the recent literature about the occurrence of both acetotrophic and hydrogenotrophic methanogens during anaerobic conversion of particulate biomass to methane (not wastewater treatment), while this review does not cover the activity of the acetate oxidizing bacteria.
Genomic and metabolic adaptations of Methanobrevibacter smithii to the human gut
Results indicate that M. smithii is well equipped to persist in the distal intestine through production of surface glycans resembling those found in the gut mucosa, and regulated expression of adhesin-like proteins, and consumption of a variety of fermentation products produced by saccharolytic bacteria, and effective competition for nitrogenous nutrient pools.
The Repertoire of Archaea Cultivated from Severe Periodontitis
These observations of living methanogens, strengthen previous observations made on DNA-based studies regarding the role of methanogenic archaea, in periodontitis.
High Prevalence of Methanobrevibacter smithii and Methanosphaera stadtmanae Detected in the Human Gut Using an Improved DNA Detection Protocol
In contrast to previous reports, these data indicate a high prevalence of the methanogens M. smithii and M. stadtmanae in the human gut, with the former being an almost ubiquitous inhabitant of the intestinal microbiome.
The aerobic activity of metronidazole against anaerobic bacteria.
Metabolic, Phylogenetic, and Ecological Diversity of the Methanogenic Archaea
The ecology of methanogens highlights their complex interactions with other anaerobes and the physical and chemical factors controlling their function.
Diversity of Human-Associated Methanobrevibacter smithii Isolates Revealed by Multispacer Sequence Typing
A multispacer sequence typing (MST) for genotyping the genetic variants of M. smithii is developed, a sequencing-based method which discriminates several genetic variants within M.smithii.