Aerobic culture of methanogenic archaea without an external source of hydrogen

@article{Khelaifia2016AerobicCO,
  title={Aerobic culture of methanogenic archaea without an external source of hydrogen},
  author={Saber Khelaifia and Jean-Christophe Lagier and Vanessa Demonfort Nkamga and Elodie Guilhot and Michel Drancourt and Didier Raoult},
  journal={European Journal of Clinical Microbiology \& Infectious Diseases},
  year={2016},
  volume={35},
  pages={985-991}
}
Culturing methanogenic archaea is fastidious, expensive, and requires an external source of hydrogen and carbon dioxide. [] Key Method To obtain colonies, we cultivated M. smithii in an agar plate in the upper part of a double chamber flask with a liquid culture of B. thetaiotaomicron in the lower compartment. We subsequently cultured four other methanogenic species for the first time and successfully isolated 13 strains of M. smithii and nine strains of Methanobrevibacter oralis from 100 stools and 45 oral…
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A polyphasic approach, including microscopic observation by fluorescence in situ hybridisation, polymerase chain reaction (PCR) sequencing detection, identification and culture, is developed to isolate and genotype M. smithii in one-day-old newborns’ gastric juice specimens.
Methanogens as emerging pathogens in anaerobic abscesses
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Background Methanobrevibacter smithii, Methanobrevibacter oralis, Methanosphaera stadtmanae, Methanomassilicoccus luminyensis and Methanobrevibacter arboriphilicus have been cultured from human
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