Aerobic culture of methanogenic archaea without an external source of hydrogen

@article{Khelaifia2016AerobicCO,
  title={Aerobic culture of methanogenic archaea without an external source of hydrogen},
  author={S. Khelaifia and J. Lagier and V. D. Nkamga and E. Guilhot and M. Drancourt and D. Raoult},
  journal={European Journal of Clinical Microbiology \& Infectious Diseases},
  year={2016},
  volume={35},
  pages={985-991}
}
Culturing methanogenic archaea is fastidious, expensive, and requires an external source of hydrogen and carbon dioxide. [...] Key Method To obtain colonies, we cultivated M. smithii in an agar plate in the upper part of a double chamber flask with a liquid culture of B. thetaiotaomicron in the lower compartment. We subsequently cultured four other methanogenic species for the first time and successfully isolated 13 strains of M. smithii and nine strains of Methanobrevibacter oralis from 100 stools and 45 oral…Expand
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