Advantages of using a cell separator and metrizamide gradients for human islet purification.

@article{Vargas1996AdvantagesOU,
  title={Advantages of using a cell separator and metrizamide gradients for human islet purification.},
  author={Fabian Santiago Vargas and Marta Vives-Pi and N. Somoza and Leandro Alcalde and Pere Armengol and Merc{\`e} Mart{\'i} and Laurence Serradell and M. Costa and Jaume Fern{\'a}ndez-Llamazares and Anna M Sanmart{\'i} and Ricardo Pujol-Borrell},
  journal={Transplantation},
  year={1996},
  volume={61 11},
  pages={1562-6}
}
Human islet transplantation has a high rate of failure, often due to primary nonfunction, which suggests that islets are damaged during the processing of the pancreas. The preparation of human islets for transplantation is still a complex process that requires large teams of surgical and laboratory personnel. To overcome this problem, we have adopted the use of the IBM 2991 COBE cell separator and a metrizamide/Ficoll density medium that is easy to prepare. Twenty-seven pancreatic glands have… CONTINUE READING