Advantages of branched peptides in serodiagnosis. Detection of HIV-specific antibodies and the use of glycine spacers to increase sensitivity.

@article{Marsden1992AdvantagesOB,
  title={Advantages of branched peptides in serodiagnosis. Detection of HIV-specific antibodies and the use of glycine spacers to increase sensitivity.},
  author={Howard S. Marsden and Ania M. Owsianka and Susan Y Graham and Gordon W. McLean and Cliff A Robertson and John H. Subak-Sharpe},
  journal={Journal of immunological methods},
  year={1992},
  volume={147 1},
  pages={65-72}
}
The reactivities of antibodies with branched and monomeric peptides were compared in ELISA assays. We found that lower amounts of antibodies could be detected with branched peptides than with monomeric peptides. This was observed with a monoclonal antibody and with antibodies in the sera of various HIV-positive individuals. To investigate the physical aspects of branched peptides important for the observed increase in sensitivity, glycine spacers of different lengths were introduced between the… CONTINUE READING

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