Advantages and disadvantages of conditional systems for characterization of essential genes in Toxoplasma gondii.

@article{JimnezRuiz2014AdvantagesAD,
  title={Advantages and disadvantages of conditional systems for characterization of essential genes in Toxoplasma gondii.},
  author={Elena Jim{\'e}nez-Ruiz and Eleanor H. Wong and Gurman Singh Pall and Markus Meissner},
  journal={Parasitology},
  year={2014},
  volume={141 11},
  pages={1390-8}
}
The dissection of apicomplexan biology has been highly influenced by the genetic tools available for manipulation of parasite DNA. Here, we describe different techniques available for the generation of conditional mutants. Comparison of the advantages and disadvantages of the three most commonly used regulation systems: the tetracycline inducible system, the regulation of protein stability and site-specific recombination are discussed. Using some previously described examples we explore some of… CONTINUE READING
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