Advancing the functional utility of PAR-CLIP by quantifying background binding to mRNAs and lncRNAs

@inproceedings{Friedersdorf2013AdvancingTF,
  title={Advancing the functional utility of PAR-CLIP by quantifying background binding to mRNAs and lncRNAs},
  author={Matthew B. Friedersdorf and Jack D. Keene},
  booktitle={Genome Biology},
  year={2013}
}
Sequence specific RNA binding proteins are important regulators of gene expression. Several related crosslinking-based, high-throughput sequencing methods, including PAR-CLIP, have recently been developed to determine direct binding sites of global protein-RNA interactions. However, no studies have quantitatively addressed the contribution of background binding to datasets produced by these methods. We measured non-specific RNA background in PAR-CLIP data, demonstrating that covalently… CONTINUE READING
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