Advances in real-time PCR: application to clinical laboratory diagnostics.

  title={Advances in real-time PCR: application to clinical laboratory diagnostics.},
  author={Bernhard Kaltenboeck and Chengming Wang},
  journal={Advances in clinical chemistry},
The polymerase chain reaction (PCR) has become one of the most important tools in molecular diagnostics, providing exquisite sensitivity and specificity for detection of nucleic acid targets. Real-time monitoring of PCR has simplified and accelerated PCR laboratory procedures and has increased information obtained from specimens including routine quantification and differentiation of amplification products. Clinical diagnostic applications and uses of real-time PCR are growing exponentially… CONTINUE READING
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