Advances in molecular diagnostic testing for central nervous system infections

  title={Advances in molecular diagnostic testing for central nervous system infections},
  author={Catherine F Houlihan and Tehmina Bharucha and Judith Breuer},
  journal={Current Opinion in Infectious Diseases},
Purpose of review Central nervous system (CNS) infections present an ongoing diagnostic challenge for clinicians, with an aetiological agent remaining unidentified in the majority of cases even in high-income settings. This review summarizes developments in a range of diagnostic methods published in the past 18 months. Recent findings Several commercial assays exist for the detection of viral, bacterial and fungal pathogens using single multiplex PCR. Multicentre validation of the Biofire… 

Acute bacterial meningitis

Clinicians must remain vigilant for bacterial meningitis, as constantly changing epidemiology and emerging pathogenesis data are increasing the understanding of meneditis.

Impact of cerebrospinal fluid syndromic testing in the management of children with suspected central nervous system infection

The use of FA-ME was able to reduce significantly the use of antimicrobials, the hospitalization days, and the total cost comparing to the control group in children with suspected CNS infection.

Trends and Developments in the Detection of Pathogens in Central Nervous System Infections: A Bibliometric Study

Exploring more advanced laboratory assays to improve the diagnostic accuracy of pathogens is essential for CNS infection research, and metagenomic next-generation sequencing is emerging as a novel useful unbiased approach for diagnosing infectious diseases of the CNS.

Cerebrospinal fluid analysis: current diagnostic methods in central nervous system infectious diseases.

  • H. Gomes
  • Biology, Medicine
    Arquivos de neuro-psiquiatria
  • 2022
In this article, some recent diagnostic methods applied to CSF analysis for frequent CNS infections are presented.

Faster infection diagnostics for intensive care unit (ICU) patients

This review of recent literature identified diagnostic methods for the most prevalent infections in these patients through a search of the databases PubMed, evidence-based medicine online, York University reviewers group, Cochrane, MBE-Trip, and Sumsearch using the terms: adult, clinical laboratory techniques, critical care, early diagnosis, microbiology, molecular diagnostic techniques, spectrometry and metagenomics.

Comparison of the accuracy of CapitalBio Mycobacterium real-time polymerase chain reaction and Xpert MTB/RIF for the diagnosis of tuberculous meningitis.

Diagnosis of Invasive Fungal Infection in Patients With Solid Organ Transplants: How to Approach?

In this review, epidemiological characteristics, clinical course, risk factors and diagnostic tests of common IFIs in solid organ transplant recipients are discussed.

Limitation of ctrA as a Target for Neisseria meningitidis Identification and Potential Alternative Targets

This paper presents a meta-analysis of 148 cases of Clostridium difficile infection in eight Canadian hospitals over a 12-month period in the period of May 21 to 29, 1997 and shows clear patterns of infection and progression.




Evaluation of a Commercial Multiplex Molecular Panel for Diagnosis of Infectious Meningitis and Encephalitis

The Film arrays offered a rapid (∼60-min), syndrome-based approach for the detection of select meningitis and encephalitis pathogens and low overall agreement between the ME panel and antigen testing for detection of C. neoformans/C.

Virological Diagnosis of Central Nervous System Infections by Use of PCR Coupled with Mass Spectrometry Analysis of Cerebrospinal Fluid Samples

It is demonstrated that PCR-MS analysis is a valuable tool to identify common neurotropic viruses in CSF (with, however, limitations that were identified regarding EBV and EV detection) and may be of major interest in better understanding the clinical impact of multiple or neglected viral neurological infections.

Metagenomic next-generation sequencing as a diagnostic tool for toxoplasmic encephalitis

The use of metagenomic NGS to search for an infectious etiology in a human immunodeficiency virus (HIV)-infected patient with lethally diffuse brain lesions and sequencing detection identified 65,357 sequence reads uniquely aligned to the Toxoplasma gondii genome suggests that metagenomics NGS may be a useful diagnostic tool for toxoplasmic encephalitis.

Impact of Cerebrospinal Fluid Multiplex Assay on Diagnosis and Outcomes of Central Nervous System Infections in Children: A Before and After Cohort Study

The multiplex assay is a useful adjunct diagnostic tool enabling prompt organism identification and reducing antibiotic treatment and hospitalization duration and would be of most value to hospitals that do not have access to an onsite molecular laboratory.

Evaluation of a TaqMan Array Card for Detection of Central Nervous System Infections

A TaqMan array card (TAC) that detects multiple pathogens simultaneously from cerebrospinal fluid and may be useful for outbreak investigation and surveillance of suspected neurological disease.

Chronic Meningitis Investigated via Metagenomic Next-Generation Sequencing

Diverse microbial pathogens were identified by mNGS in the CSF of patients with diagnostically challenging subacute or chronic meningitis, including a case of subarachnoid neurocysticercosis that defied diagnosis for 1 year, and the first reported case of CNS vasculitis caused by Aspergillus oryzae.