Advances in light microscopy for neuroscience.

  title={Advances in light microscopy for neuroscience.},
  author={Brian A Wilt and Laurie D. Burns and Eric Tatt Wei Ho and Kunal K. Ghosh and Eran A. Mukamel and Mark J. Schnitzer},
  journal={Annual review of neuroscience},
Since the work of Golgi and Cajal, light microscopy has remained a key tool for neuroscientists to observe cellular properties. Ongoing advances have enabled new experimental capabilities using light to inspect the nervous system across multiple spatial scales, including ultrastructural scales finer than the optical diffraction limit. Other progress permits functional imaging at faster speeds, at greater depths in brain tissue, and over larger tissue volumes than previously possible. Portable… 
In vivo optical microendoscopy for imaging cells lying deep within live tissue.
In vivo optical microendoscopy using gradient refractive index (GRIN) microlenses that can be inserted into tissue is discussed, allowing imaging across a range of spatial scales and with spatial resolution that can now closely approach that offered by standard water-immersion microscope objectives.
Combining Confocal Laser Scanning Microscopy with Serial Section Reconstruction in the Study of Adult Neurogenesis
A method to combine confocal laser scanning microscopy and serial section reconstruction in order to reconstruct large volumes of brain tissue at cellular resolution is presented and the morphology and spatial organization of a group of doublecortin (DCX)+ neuroblasts located in the lateral striatum of the late post-natal guinea pig is investigated.
Knife-edge scanning microscopy for connectomics research
In this paper, we will review a novel microscopy modality called Knife-Edge Scanning Microscopy (KESM) that we have developed over the past twelve years (since 1999) and discuss its relevance to
Functional imaging in freely moving animals
Towards Optical Connectomics : Feasibility of 3 D Reconstruction of Neural Morphology Using Expansion Microscopy and In Situ Molecular Barcoding
This thesis introduces the study of the properties, via computer simulation, of a candidate automated approach to algorithmic reconstruction of dense neural morphology, based on simulated data of the kind that would be obtained via two emerging molecular technologies-expansion microscopy and in-situ molecular barcoding.
Longitudinal Two-Photon Imaging of Dorsal Hippocampal CA1 in Live Mice.
This chronic preparation can be used in combination with other techniques such as micro-endoscopy, head-mounted wide field microscopy or three-photon microscopy, thus greatly expanding the toolbox to study cellular and network processes involved in learning and memory.
Super-Resolution Microscopy Techniques in the Neurosciences
Galileo Galilei invented the first microscope “occhiolino,” by combining a concave and a convex lens in 1600s. Robert Hooke and Anton van Leeuwenhoek later modified it to look at living things. Since
Multiscale and Multimodal Imaging for Connectomics
The various imaging modalities aiming for high-throughput brain imaging, as well as the challenges encountered in imaging the connectome are discussed.
Advanced Imaging Techniques
An overview of the recent data arising from studies of electron and multiphoton microscopy, in vivo microendoscopy, and hybrid molecular imaging, such as positron emission tomography (PET)/computed tomography and PET/magnetic resonance and multimodality tracers is provided.


Live-cell imaging of dendritic spines by STED microscopy
It is shown that time lapse stimulated emission depletion imaging of dendritic spines of YFP-positive hippocampal neurons in organotypic slices outperforms confocal microscopy in revealing important structural details.
In vivo multiphoton microscopy of deep brain tissue.
It is shown that multiphoton microscopy through GRIN lenses enables minimally invasive, subcellular resolution several millimeters in the anesthetized, intact animal, and it is presented in vivo images of cortical layer V and hippocampus in theAnesthetizing Thy1-YFP line H mouse.
Second-harmonic imaging microscopy for visualizing biomolecular arrays in cells, tissues and organisms
Recent studies of the three-dimensional in vivo structures of well-ordered protein assemblies, such as collagen, microtubules and muscle myosin, are beginning to establish SHIM as a nondestructive imaging modality that holds promise for both basic research and clinical pathology.
Optical Sectioning Deep Inside Live Embryos by Selective Plane Illumination Microscopy
Large, living biological specimens present challenges to existing optical imaging techniques because of their absorptive and scattering properties. We developed selective plane illumination
Markers for correlated light and electron microscopy.
All-Optical Histology Using Ultrashort Laser Pulses
A Miniature Head-Mounted Neurotechnique Two-Photon Microscope: High-Resolution Brain Imaging in Freely Moving Animals
Two-photon imaging is extended from anes- confined to the focal plane, permitting efficient fluoresthetized, head-stabilized to awake, freely moving rats and stable imaging the question arising whether in unrestrained animals was possible except during sudden head movements is possible.
SLM Microscopy: Scanless Two-Photon Imaging and Photostimulation with Spatial Light Modulators
A “scanless” microscope that uses a diffractive spatial light modulator (SLM) to shape an incoming two-photon laser beam into any arbitrary light pattern, which allows the simultaneous imaging or photostimulation of different regions of a sample with three-dimensional precision.