Advances in laboratory evolution of enzymes.

@article{Bershtein2008AdvancesIL,
  title={Advances in laboratory evolution of enzymes.},
  author={Shimon Bershtein and Dan S. Tawfik},
  journal={Current opinion in chemical biology},
  year={2008},
  volume={12 2},
  pages={
          151-8
        }
}
We address recent developments in the area of laboratory, or directed evolution, with a focus on enzymes and on new methodologies of generic potential. We survey three main areas: (i) library making techniques, including the application of computational and rational methods for library design; (ii) screening and selection techniques, including recent applications of enzyme screening by FACS (fluorescence activated cell sorter); (iii) new approaches for performing directed evolution, and in… CONTINUE READING
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