Advances in cell-free protein synthesis for the functional and structural analysis of membrane proteins.

@article{Junge2011AdvancesIC,
  title={Advances in cell-free protein synthesis for the functional and structural analysis of membrane proteins.},
  author={Friederike Junge and Stefan Haberstock and Christian Roos and Susanne Stefer and Davide Proverbio and Volker D{\"o}tsch and Frank Bernhard},
  journal={New biotechnology},
  year={2011},
  volume={28 3},
  pages={262-71}
}
Cell-free expression has emerged as a powerful technique to overcome major restrictions of classical in vivo membrane protein production, with sample yields of mgms of protein per ml reaction volume possible in less than a day. The open nature and high versatility of cell-free expression allows a variety of completely new ways to rationally design and optimise expression environments as well as to modulate folding kinetics for membrane proteins independent of their origin, size, topology and… CONTINUE READING

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