Corpus ID: 33382178

Advances in ancient DNA studies

@inproceedings{Caramelli2006AdvancesIA,
  title={Advances in ancient DNA studies},
  author={David Caramelli and Giampietro Lago},
  year={2006}
}
The first aDNA studies used bacterial cloning to amplify small sequences retrieved from skins of animal and human mummies, and revealed the inefficient reaction kinetics of this technique (Higuchi et al., 1984; Paabo 1985, 1989). These studies demonstrated that the genetic material surviving in ancient specimens was often principally microbial or fungal in origin. Endogenous DNA was generally limited to very low concentrations of short, damaged fragments of multi-copy loci such as mitochondrial… Expand

Figures from this paper

Genetics and southern African prehistory: an archaeological view.
  • P. Mitchell
  • Geography, Medicine
  • Journal of anthropological sciences = Rivista di antropologia : JASS
  • 2010
TLDR
This paper reviews these studies from an archaeological standpoint, concentrating on modern human origins, the introduction of pastoralism to southern Africa and admixture between the region's indigenous foragers and incoming Bantu-speaking farmers. Expand

References

SHOWING 1-10 OF 137 REFERENCES
Ancient DNA analysis of human populations.
TLDR
Five well-preserved skeletal specimens from the western United States dating from 800-1600 A.D. yielded DNA samples with levels of contamination ranging from 0-100%, as determined by the presence or absence of New World-specific mitochondrial markers. Expand
Spiking of contemporary human template DNA with ancient DNA extracts induces mutations under PCR and generates nonauthentic mitochondrial sequences.
TLDR
It is shown that ancient DNA extracts on their own can have an inhibitory and mutagenic effect under PCR, and induced mutations in a nonrandom fashion are found, implying that PCR-induced mutations are likely to be an intrinsic and general problem of PCR amplifications of ancient templates. Expand
The retrieval of ancient human DNA sequences.
TLDR
These results show that more experimental work than is often applied is necessary to ensure that DNA sequences amplified from ancient human remains are authentic and quantitation of the numbers of amplifiable molecules is a useful tool to determine the role of contaminating contemporary molecules and PCR errors in amplifications from ancient DNA. Expand
Ancient DNA: extraction, characterization, molecular cloning, and enzymatic amplification.
  • S. Pääbo
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences of the United States of America
  • 1989
TLDR
The polymerase chain reaction can be used to amplify and study short mitochondrial DNA sequences that are of anthropological and evolutionary significance and opens up the prospect of performing diachronical studies of molecular evolutionary genetics. Expand
DNA sequences from multiple amplifications reveal artifacts induced by cytosine deamination in ancient DNA.
TLDR
It is shown that DNA molecules amplified by PCR from DNA extracted from animal bones and teeth that vary in age between 25 000 and over 50 000 years carry C-->T and G-->A substitutions, which are due to the occurrence of modified deoxycytidine residues in the template DNA. Expand
Recovery of 16S ribosomal RNA gene fragments from ancient halite
TLDR
Terminal restriction fragment length polymorphism analyses indicate complex and different populations of microorganisms or their free DNA in ancient halites of different ages. Expand
Statistical evidence for miscoding lesions in ancient DNA templates.
TLDR
A statistical model for analyzing PCR-mediated base-misincorporations, catalyzed by the commonly used Thermus aquaticus (Taq) polymerase enzyme, in amplification products from fossil remains is presented. Expand
Authenticating DNA Extracted From Ancient Skeletal Remains
TLDR
Work with a range of Holocene skeletal material from domestic animals and humans suggests that more than 50% of skeletal remains from the past two thousand years are likely to contain amplifiable endogenous DNA, but that in the case of human material great care is needed to distinguish this from contamination introduced before the samples reach the laboratory. Expand
Chloroplast DNA sequence from a Miocene Magnolia species
TLDR
The extraction of DNA from fossil leaf samples from the Miocene Clarkia deposit, the amplification of an 820-base pair DNA fragment from the chloroplast gene rbcL from a fossil of the genus Magnolia, and its subsequent sequencing extend the ability to analyse ancient DNA and may open new avenues into problems in palaeobotany, biogeography, and in the calibration of mutation rates. Expand
Miocene DNA sequences — a dream come true?
TLDR
The ability of the PCR to amplify a few intact DNA molecules that are extracted from old tissues has for the first time made it possible to go back in time and study old DNA sequences, and the PCR can even recreate longer sequences from short fragments in the test tube. Expand
...
1
2
3
4
5
...