Advances in Single-Particle Electron Cryomicroscopy Structure Determination applied to Sub-tomogram Averaging

@inproceedings{Bharat2015AdvancesIS,
  title={Advances in Single-Particle Electron Cryomicroscopy Structure Determination applied to Sub-tomogram Averaging},
  author={Tanmay A. M. Bharat and Christopher J. Russo and Jan L{\"o}we and Lori A Passmore and Sjors H. W. Scheres},
  booktitle={Structure},
  year={2015}
}
Recent innovations in specimen preparation, data collection, and image processing have led to improved structure determination using single-particle electron cryomicroscopy (cryo-EM). Here we explore some of these advances to improve structures determined using electron cryotomography (cryo-ET) and sub-tomogram averaging. We implement a new three-dimensional model for the contrast transfer function, and use this in a regularized likelihood optimization algorithm as implemented in the RELION… CONTINUE READING
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