Advances in 2-phenylethanol production from engineered microorganisms.

@article{Wang2019AdvancesI2,
  title={Advances in 2-phenylethanol production from engineered microorganisms.},
  author={Yuqin Wang and H. Zhang and Xinyao Lu and H. Zong and B. Zhuge},
  journal={Biotechnology advances},
  year={2019},
  volume={37 3},
  pages={
          403-409
        }
}
2-Phenylethanol (2-PE) is an important flavor ingredient with a rose-like odor. Due to concerns about the toxic byproducts potentially found in 2-PE from chemical synthesis, consumers prefer the natural aroma compound, promoting the biosynthesis of 2-PE. Various microorganisms produce 2-PE naturally with low yield. Recent metabolic engineering strategies in yeasts and Escherichia coli have achieved great success in improving 2-PE bioproduction, including the alleviation of feed-back inhibition… Expand
Enhancement of 2-phenylethanol production by a wild-type Wickerhamomyces anomalus strain isolated from rice wine.
TLDR
The results showed that the ability was expanded to harness the Ehrlich pathway for the production of high-value aromatics in aroma-producing yeast species in 2-PE production with W. anomalus. Expand
Valorization of agro-industrial wastes by producing 2-phenylethanol via solid-state fermentation: Influence of substrate selection on the process.
TLDR
The results suggest that the desirable traits a solid media needs for promoting 2-PE production via SSF could be reached by using a combination of wastes in a synergistic approach. Expand
Genetic engineering of an industrial yeast Candida glycerinogenes for efficient production of 2-phenylethanol.
TLDR
This study combined metabolic engineering and tolerance engineering strategies to maximize the valuable rose-smell 2-phenylethanol (2-PE) production in Candida glycerinogenes, a GRAS diploid industrial yeast. Expand
Efficient synthesis of 2-phenylethanol from L-phenylalanine by engineered Bacillus licheniformis using molasses as carbon source
TLDR
This study develops a robust strain as well as the cost-efficient process for high-level production of 2-phenylethanol production, which lays a substantial foundation for industrial production of 1-phenylalanine and Bacillus licheniformis. Expand
Sensing, Uptake and Catabolism of L-Phenylalanine During 2-Phenylethanol Biosynthesis via the Ehrlich Pathway in Saccharomyces cerevisiae
TLDR
Current progress on the signal transduction pathways of L-Phe sensing, uptake of extracellular L-phe and 2-PE synthesis from L- Phe through the Ehrlich pathway are reviewed. Expand
Saccharomyces Cerevisiae Strains Selected From Nature Significantly Increased the Production of 2-Phenylethanol Through Protoplast Fusion
  • Lucheng Lin, Zhiwei Xu, Weixia Wang, Kun Wang, Tingheng Zhu
  • Chemistry
  • 2021
Background: 2-Phenylethanol (2-PE) is an aromatic alcohol with rose fragrance, which is widely used as an additive in food, tobacco and daily chemical industries. Yeast is the main microorganismExpand
2-phenylethanol (rose aroma) production potential of an isolated pichia kudriavzevii through solid-state fermentation
Abstract 2-phenylethanol (2-PE) is a higher alcohol widely used in industry that can be obtained by solid-state fermentation (SSF) using low-cost raw materials. This report describes the 2-PEExpand
Adaptive responses of Kluyveromyces marxianus CCT 7735 to 2-phenylethanol stress: alterations in membrane fatty-acid composition, ergosterol content, exopolysaccharide production and reduction in reactive oxygen species.
TLDR
2-PE stress impaired yeast growth, glucose uptake, fermentative metabolism, membrane permeability, and cell viability, and changes in membrane fatty-acid composition, ergosterol content, exopolysaccharides production and reduction of the ROS levels appear to be the result of adaptive responses in K. marxianus. Expand
Connecting central carbon and aromatic amino acid metabolisms to improve de novo 2-phenylethanol production in Saccharomyces cerevisiae.
TLDR
It is demonstrated that improving phosphoenolpyruvate supply by expressing pyruvates kinase variants and eliminating the formation of p-hydroxy-phenylethanol without creating tyrosine auxotrophy significantly contributed to improve 2PE production in S. cerevisiae. Expand
Refactoring Ehrlich Pathway for High-Yield 2-Phenylethanol Production in Yarrowia lipolytica
TLDR
The reported work expands the ability to harness the Ehrlich pathway for production of high-value aromatics in oleaginous yeast species by improving 2-oxoglutarate trafficking across the mitochondria membrane and constructing a cytosolic aKG source pathway. Expand
...
1
2
3
...

References

SHOWING 1-10 OF 60 REFERENCES
Recent advances in biotechnological production of 2-phenylethanol.
  • D. Hua, P. Xu
  • Chemistry, Medicine
  • Biotechnology advances
  • 2011
TLDR
Recent advances in biotechnological production of 2-PE are reviewed, with emphasis on the strategies used to increase production and the applications of in situ product removal techniques. Expand
Biotechnological production of 2-phenylethanol
TLDR
This review describes the microbial production of 2-PE, and also summarizes the chemical syntheses and the market situation. Expand
Bioconversion of L-phenylalanine to 2-phenylethanol by the novel stress-tolerant yeast Candida glycerinogenes WL2002-5
TLDR
Results suggest C. glycerinogenes WL2002-5 is a robust strain for the bioproduction of 2-PE with potential for commercial exploitation. Expand
Bioprocesses for 2-phenylethanol and 2-phenylethyl acetate production: current state and perspectives
TLDR
This review compiles the most recent developments in the biotechnological production of 2-PE and2-PEA, indicating the most studied strains producing 2- PE and 2-peA, the current advances in the in situ product recovery in liquid systems, an overview of the strain developments, and the progress in the use of residue-based systems. Expand
Enhanced 2-phenylethanol production from L-phenylalanine via in situ product adsorption
Abstract Biotransformation of L-phenylalanine (L-Phe) to 2-phenylethanol (2-PE) was limited by product cytotoxicity. In order to improve 2-PE production, a biotransformation strategy applying in situExpand
Screening of yeasts for the production of the aroma compound 2-phenylethanol in a molasses-based medium
TLDR
Fourteen yeast strains were screened for production of 2-phenylethanol from l-phenylalanine with molasses as carbon source and the most productive strains were Kluyveromyces marxianus CBS 600 and CBS 397. Expand
A continuous and adsorptive bioprocess for efficient production of the natural aroma chemical 2-phenylethanol with yeast.
TLDR
A continuous approach using macroporous resin as in situ adsorbent made it possible that the quantity and viability of the cells were improved simultaneously and the highest space-time yield reported so far was obtained. Expand
Improving 2-Phenylethanol Production via Ehrlich Pathway Using Genetic Engineered Saccharomyces cerevisiae Strains
TLDR
It is demonstrated that the 2- PE production was significantly improved by enhanced expression of the two key enzymes encoded by ARO8 and ARO10 in the Ehrlich pathway, providing new perspectives for enhancing “natural” 2-PE production in S. cerevisiae. Expand
Biosynthesis of 2-phenylethanol from glucose with genetically engineered Kluyveromyces marxianus.
TLDR
K Kluyveromyces marxianus was genetically engineered for overproduction of 2-PE from glucose by overexpressing phenylpyruvate decarboxylase and alcohol dehydrogenase genes of Saccharomyces cerevisiae and aroG from Klebsiella pneumoniae encoding a feedback resistant mutant of 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase was overexpressed. Expand
Yeast factories for the production of aromatic compounds: from building blocks to plant secondary metabolites
TLDR
This review contains a collection of state-of-the-art metabolic engineering work devoted towards unraveling the mechanisms for enhancing the flux of carbon into the aromatic pathway. Expand
...
1
2
3
4
5
...