Advances and Clinical Practice in Pyrosequencing

@inproceedings{Zhou2016AdvancesAC,
  title={Advances and Clinical Practice in Pyrosequencing},
  author={Guohua Zhao Jing Zhou and Qinxin Song},
  booktitle={Springer Protocols Handbooks},
  year={2016}
}
To establish a simple method for preparing single-stranded DNA templates for pyrosequencing, the Linearafter-the-exponential (LATE)-PCR technology on the basis of Taq DNA polymerase without hotstart capacity was applied to amplify a 78-bp sequence (containing the SNP6 site), and the PCR-enhancing reagents (glycerol and BSA) were used to increase the effi ciency and specialization, much more before the reagents A and B were designed to eliminate the impurity (limited primers, PPi, dNTPs, and so… CONTINUE READING

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