Advanced techniques for in situ analysis of the biofilm matrix (structure, composition, dynamics) by means of laser scanning microscopy.

Abstract

The extracellular constituents in bioaggregates and biofilms can be imaged four dimensionally by using laser scanning microscopy. In this protocol we provide guidance on how to examine the various extracellular compartments in between microbial cells and communities associated with interfaces. The current options for fluorescence staining of matrix… (More)
DOI: 10.1007/978-1-4939-0467-9_4

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@article{Neu2014AdvancedTF, title={Advanced techniques for in situ analysis of the biofilm matrix (structure, composition, dynamics) by means of laser scanning microscopy.}, author={Thomas R. Neu and John Todd R. Lawrence}, journal={Methods in molecular biology}, year={2014}, volume={1147}, pages={43-64} }