Advanced Application of the In Vivo Cryotechnique to Immunohistochemistry for Animal Organs

@inproceedings{Ohno2004AdvancedAO,
  title={Advanced Application of the In Vivo Cryotechnique to Immunohistochemistry for Animal Organs},
  author={Nobuhiko Ohno and Nobuo Terada and Shinichi Ohno},
  year={2004}
}
The quick-freezing method often used for physical fixation also contributed enormously to the advancement of morphology, but it could not provide enough information about the dynamic morphological changes in vivo in living animal organs. Therefore, we developed the in vivo cryotechnique in 1995, which could directly cryofix organs in vivo under anesthetized conditions without stopping blood circulation or producing effects of anoxia. We have already reported new findings about the in vivo… CONTINUE READING

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Immunohistochemical analyses on serum proteins in nephrons of protein-overload mice by "in vivo cryotechnique".

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  • 2007
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