Adult ventricular cardiomyocytes: isolation and culture.

  title={Adult ventricular cardiomyocytes: isolation and culture.},
  author={Klaus-Dieter Schl{\"u}ter and Daniela Schreiber},
  journal={Methods in molecular biology},
Isolated cardiomyocytes are a prerequisite to study the biology of cardiomyocytes. Efficient isolation is difficult, as these cells adhere firmly together in the heart and do not divide. Therefore, any experiment is restricted to the amount of calcium-tolerant, rod-shaped cardiomyocytes that can be initially isolated from the heart. This chapter gives detailed instructions on how ventricular cardiomyocytes can be isolated from an intact adult heart. The method is based on the principle of… CONTINUE READING

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