Adult muscle 'stem' cells can be sustained in culture as free-floating myospheres.

  title={Adult muscle 'stem' cells can be sustained in culture as free-floating myospheres.},
  author={Karen A. Westerman and Ashley R. Penvose and Zhong Yang and Paul D. Allen and Charles A. Vacanti},
  journal={Experimental cell research},
  volume={316 12},
The effectiveness of cell-based therapy to treat muscle disease has been hampered by difficulties in isolating, maintaining and propagating the stem cells that are needed for treatment. Here we report the isolation of muscle-derived stem cells from both young and old mice and their propagation over extended periods of time in culture as "free-floating" myospheres. Analysis of these sphere-forming cells showed that they express stem cell antigen-1 (Sca-1), beta1 integrin (CD29), Thy-1 (CD90… CONTINUE READING

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