Adult mice generated from induced pluripotent stem cells

  title={Adult mice generated from induced pluripotent stem cells},
  author={Michael J. Boland and Jennifer L. Hazen and Kristopher L. Nazor and Alberto R Rodriguez and Wesley D. Gifford and Greg Martin and Sergey Kupriyanov and Kristin K. Baldwin},
Recent landmark experiments have shown that transient overexpression of a small number of transcription factors can reprogram differentiated cells into induced pluripotent stem (iPS) cells that resemble embryonic stem (ES) cells. These iPS cells hold great promise for medicine because they have the potential to generate patient-specific cell types for cell replacement therapy and produce in vitro models of disease, without requiring embryonic tissues or oocytes. Although current iPS cell lines… 

Viable Fertile Mice Generated from Fully Pluripotent iPS Cells Derived from Adult Somatic Cells

Comparison of iPS cells from embryonic origin (MEF), progenitor cells (neural stem cells) or differentiated somatic cells (TTF) reveals that fully pluripotent developmental potential can be reached by each cell type, although with different induction efficiencies.

Pluripotency of induced pluripotent stem cells

It is proposed in this review that further comprehensive studies of both mouse and human iPS cells are required so that additional information will be available for evaluating the quality of human i PS cells.

Pluripotent Stem Cells and Reprogrammed Cells in Farm Animals

This review summarizes the present state of the art in the derivation and maintenance of pluripotent stem cells in domestic animals.

Generation of germ cells in vitro in the era of induced pluripotent stem cells

These findings illustrate not only that iPSCs are developmentally similar to embryonic stem cells (ESCs), but also that somatic cells from adult tissues can produce gametes in vitro, that is, if they are reprogrammed into iPSC.

Analysis of Human and Mouse Reprogramming of Somatic Cells to Induced Pluripotent Stem Cells. What Is in the Plate?

It is proposed that the study of expression of bivalent domain-containing genes, which are normally silenced in ESCs, gives a valuable indication of the quality of the iPSC line, and could be used to select the best iPSCs lines out of a large number of lines generated in each reprogramming experiment.

Induced Pluripotent Stem Cells-A New Foundation in Medicine.

  • G. T. Huang
  • Biology
    Journal of experimental and clinical medicine
  • 2010


iPS cell genesis being the result of a transient gene therapy mechanism, the transient epigenetic gene therapy arm of the proposed universal stem cell gene therepy platform is presented together with concurrent approaches mediated by protein transduction, mRNA transfection and small molecules.

EMBRYONIC STEM CELLS / INDUCED PLURIPOTENT STEM CELLS Brief Report : Combined Chemical Treatment Enables Oct 4-Induced Reprogramming from Mouse Embryonic Fibroblasts

It has been established that exogenous expression of four transcription factors (Oct4, Klf4, Sox2, and c-Myc) can reprogram mammalian somatic cells to pluripotent states. Further studies demonstrated

Generation of induced pluripotent stem cells by reprogramming mouse embryonic fibroblasts with a four transcription factor, doxycycline inducible lentiviral transduction system.

The protocol for reprogramming mouse embryonic fibroblast (MEF) cells with the Stemgent DOX Inducible Mouse TF Lentivirus Set is demonstrated, capable of inducing a pluripotent stem cell state that displays the pluripotency markers characteristic of ES cells.

Complete reprogramming to all-iPS mice

It is shown that iPS cells can directly produce viable mice through tetraploid complementation, the most rigorous test of totalipotency, which is used as the ultimate test in demonstrating the totalipOTency of ES cells to contribute to every cell lineage in a future embryo.



Generation of human-induced pluripotent stem cells

This protocol describes how to establish primary human fibroblasts lines and how to derive iPS cells by retroviral transduction of reprogramming factors, and takes 2 months to complete reprograming human primary fibro Blasts starting from biopsy.

Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells

Somatic cell nuclear transfer allows trans-acting factors present in the mammalian oocyte to reprogram somatic cell nuclei to an undifferentiated state. We show that four factors (OCT4, SOX2, NANOG,

Derivation of completely cell culture-derived mice from early-passage embryonic stem cells.

Fully potent early passage R1 cells and the R1-S3 subclone should be very useful not only for ES cell-based genetic manipulations but also in defining optimal in vitro culture conditions for retaining the initial totipotency of ES cells.

Generation of transgene-free induced pluripotent mouse stem cells by the piggyBac transposon

P piggyBac transposon–based reprogramming may be used to generate therapeutically applicable iPSCs and could be identified by negative selection.

A drug-inducible transgenic system for direct reprogramming of multiple somatic cell types

It is demonstrated that various induction levels of the reprogramming factors can induce pluripotency, the duration of transgene activity directly correlates with reprograming efficiency, and cells from many somatic tissues can be reprogrammed and different cell types require different induction levels.