Adsorption of the decapeptide Cetrorelix depends both on the composition of dissolution medium and the type of solid surface.

@article{Grohganz2004AdsorptionOT,
  title={Adsorption of the decapeptide Cetrorelix depends both on the composition of dissolution medium and the type of solid surface.},
  author={Holger Grohganz and Matthias Dr Rischer and Martin Brandl},
  journal={European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences},
  year={2004},
  volume={21 2-3},
  pages={
          191-6
        }
}
  • H. Grohganz, M. Rischer, M. Brandl
  • Published 1 February 2004
  • Chemistry, Medicine
  • European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences
High performance liquid chromatography (HPLC) analysis of increasing amounts of the decapeptide Cetrorelix, a potent antagonist of the luteinising hormone-releasing hormone, in distilled water resulted in a poor and variable response when solutions of low concentration (0.2-4microg/ml) were analysed. Rinsing experiments revealed loss of analyte due to adsorption to the vial surfaces as the main reason for this. The adsorption of Cetrorelix was found to follow a Langmuir isotherm reaching a… 
Surface and Solvent Dependent Adsorption of Three Neuromedin-Like Peptides in Glass and Plastic Syringes
In the last decades, there is an increasing interest in the use of biomolecules, such as peptides, as therapeutic agents. One important challenge inherent to peptides and proteins is the occurrence
Strategies to reduce aspecific adsorption of peptides and proteins in liquid chromatography-mass spectrometry based bioanalyses: an overview.
TLDR
This work aims to present different strategies which can be generically applied to reduce nonspecific binding of peptides and proteins and focuses on strategies to efficiently reduce adsorption at components of the autosampler, column and mass spectrometer.
High-performance liquid chromatography as a technique to determine protein adsorption onto hydrophilic/hydrophobic surfaces.
TLDR
Evaluating the potential of simple high performance liquid chromatography setup for quantification of adsorbed proteins on various type of plane substrates with limited area found a decrease of peak intensity of bovine serum albumin as well as the fraction of dimer and oligomer after contact with PDMS and glass surface.
Development and in vitro evaluation of a liposome based implant formulation for the decapeptide cetrorelix.
  • H. Grohganz, I. Tho, M. Brandl
  • Chemistry, Medicine
    European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V
  • 2005
TLDR
Sustained release of therapeutically relevant CXA doses over up to 6 weeks appears feasible and vesicular phospholipid gels are considered as a promising new approach for the sustained release of peptide hormones.
Investigations on protein adsorption to coated glass vials
It was the aim of this study to investigate the adsorption of IgG1 and the model protein lysozyme to type I borosilicate glass vials and siliconized vials. In detail, the influence of the formulation
Adsorption of peptides at the sample drying step: influence of solvent evaporation technique, vial material and solution additive.
TLDR
Applying C(9)-Glu as an additive along with choosing appropriate vial type can avoid or diminish peptide loss during the evaporation procedure.
Reducing adsorption to improve recovery and in vivo detection of neuropeptides by microdialysis with LC-MS.
TLDR
It is concluded that peptides with both charged and hydrophobic regions require combined strategies to prevent adsorption and yield the best possible detection.
Development and Validation of a Reversed-phase HPLC Method for Assay of the Decapeptide Cetrorelix Acetate in Bulk and Pharmaceutical Dosage Forms
TLDR
A gradient reversed-phase high performance liquid chromatography method was developed for the assay of cetrorelix acetate, a synthetic decapeptide with gonadotropin-releasing hormone (GnRH) antagonistic activity used in infertility treatment and was validated with excellent specificity, sensitivity, and stability.
Surfactants enhance recovery of poorly soluble drugs during microdialysis sampling: Implications for in vitro dissolution‐/permeation‐studies
TLDR
Investigation of the applicability of a recently developed in vitro microdialysis‐sampling approach in connection with a dissolution‐/permeation (D/P) system, especially the impact of surfactants within the perfusion fluid found it promising to allow quantification of the unbound (free) drug concentration.
Quantitative bioanalysis of peptides by liquid chromatography coupled to (tandem) mass spectrometry.
TLDR
This article summarizes the numerous published LC-MS applications for the quantification of peptide analytes in biological matrices and discusses all different issues herewith concerned, including chromatographic aspects as the selection and effects of mobile and stationary phase, flow rate and temperature, as well as mass spectrometric characteristics such as ionization and detection modes, collision-induced dissociation of peptides and factors influencing the mass Spectrometric response.
...
1
2
3
4
...

References

SHOWING 1-10 OF 15 REFERENCES
The effect of pH and NaCl concentration on adsorption of β-lactoglobulin at hydrophilic and hydrophobic silicon surfaces
Abstract The effect of pH and NaCl concentration on the equilibrium adsorptive behavior of β-lactoglobulin at hydrophilic and hydrophobic silicon surfaces was studied using ellipsometry. Experimental
Erosion and controlled release properties of semisolid vesicular phospholipid dispersions.
TLDR
The results are important for the future development of vesicular phospholipid gels as sustained release therapeutic system, e.g. as implantable depot.
Influence of surfactants upon protein/peptide adsorption to glass and polypropylene
This paper explores the use of surfactants as a pharmaceutical excipient to reduce adsorptive losses of protein/peptide drugs. The predominant adsorption mechanism for protein/peptide drugs is shown
Adsorption of proteins onto polymeric surfaces of different hydrophilicities—a case study with bovine serum albumin
Abstract A radiolabeling technique was employed to study adsorption equilibrium and kinetics of bovine serum albumin (BSA) on five polymeric surfaces of different hydrophilicities and on glass. One
Adsorption of calcitonin to glass.
  • S. Law, C. Shih
  • Medicine, Chemistry
    Drug development and industrial pharmacy
  • 1999
TLDR
Results showed that the adsorption isotherms were of the Langmuir and Freundlich type, depending on pH, and the addition of nonionic surfactants such as Pluronic F68 and Tween 80 to the calcitonin solutions demonstrated inhibition of absorption and reduction of advertisersorption rate.
Interfacial adsorption and aggregation associated changes in secondary structure of human calcitonin monitored by ATR-FTIR spectroscopy.
TLDR
ATR-FTIR spectroscopy provides a sensitive analytical tool suggested to monitor interfacial adsorption and aggregation phenomena also of other peptides and proteins.
Morphology of semisolid aqueous phosphatidylcholine dispersions, a freeze fracture electron microscopy study
Semisolid aqueous dispersions of soy phosphatidylcholine (PC) with high PC mass fractions between 0.35 and 0.6 were prepared using a high-pressure homogeniser. Their morphologies were investigated by
Adsorption of insulin on metal surfaces in relation to association behavior
Abstract The adsorption of insulin on metal surfaces from aqueous solutions was monitored by in situ ellipsometry. Clean (hydrophilic) chromium and titanium surfaces as well as chromium surfaces
Effects of Amino Acid Composition on Protein Adsorption
Abstract The adsorption at silica and methylated silica of genetically engineered derivatives of Z and its dimer ZZ, where Z is a hydrophilic synthetic IgG binding domain derived from staphylococcal
Adsorption of proteins onto glass surfaces and its effect on the intensity of circular dichroism spectra.
TLDR
Adsorption loss of proteins onto glass surfaces is common and may lead to serious errors in experimental results, which can be minimized by using plastic containers and pipet tips, coating the cell with silicone, and wetting the cell before adding the protein solution.
...
1
2
...