Adsorption of plasmid DNA to mineral surfaces and protection against DNase I.

@article{Romanowski1991AdsorptionOP,
  title={Adsorption of plasmid DNA to mineral surfaces and protection against DNase I.},
  author={G Romanowski and Michael G. Lorenz and Wilfried Wackernagel},
  journal={Applied and environmental microbiology},
  year={1991},
  volume={57 4},
  pages={1057-61}
}
The adsorption of [3H]thymidine-labeled plasmid DNA (pHC314; 2.4 kb) of different conformations to chemically pure sand was studied in a flowthrough microenvironment. The extent of adsorption was affected by the concentration and valency of cations, indicating a charge-dependent process. Bivalent cations (Mg2+, Ca2+) were 100-fold more effective than monovalent cations (Na+, K+, NH4+). Quantitative adsorption of up to 1 microgram of negatively supercoiled or linearized plasmid DNA to 0.7 g of… CONTINUE READING
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