Adsorption of human tear lipocalin to human meibomian lipid films.

  title={Adsorption of human tear lipocalin to human meibomian lipid films.},
  author={Thomas James Millar and Poonam Mudgil and Igor A. Butovich and Chendur K Palaniappan},
  journal={Investigative ophthalmology & visual science},
  volume={50 1},
PURPOSE Tear lipocalin (Tlc) is a major lipid binding protein in tears and is thought to have an important role in stabilizing the Meibomian lipid layer by transferring lipids to it from the aqueous layer or ocular surface, or by adsorbing to it directly. These possible roles have been investigated in vitro using human Tlc. METHODS Tlc was purified from human tears by size exclusion chromatography followed by ion exchange chromatography. Three additional samples of the Tlc were prepared by… CONTINUE READING