Adsorption and reactions of chitinase and lysozyme on chitin
@article{Skujins2005AdsorptionAR, title={Adsorption and reactions of chitinase and lysozyme on chitin}, author={John Skujins and A. H. Pukite and A. D. Mclaren}, journal={Molecular and Cellular Biochemistry}, year={2005}, volume={2}, pages={221-228} }
SummaryIsotherms for adsorption of chitinase on chitin and lysozyme on chitin have been determined at two temperatures and rates of hydrolysis of chitin catalysed by these enzymes have been measured at three temperatures and at several enzyme concentrations for each. Ribonuclease, not an enzyme for chitin, and heat-denatured lysozyme and chitinase show reduced or no adsorption to this substrate.Initial hydrolysis rates of chitin by both enzymes are proportional to total enzyme concentrations in…
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References
SHOWING 1-10 OF 19 REFERENCES
THE HYDROLYSIS OF CHITIN BY CONCENTRATED HYDROCHLORIC ACID, AND THE PREPARATION OF LOW-MOLECULAR-WEIGHT SUBSTRATES FOR LYSOZYME.
- Chemistry, BiologyBiochimica et biophysica acta
- 1964
Studies on Chitin
- Biology
- 1955
There is only a weak bonding between the chitin and the water-soluble cuticular protein, and an increase to pH 9 is necessary before all the adsorbed protein can be removed.
Studies on chitin. VI. The nature of alpha- and beta-chitins.
- ChemistryAustralian journal of biological sciences
- 1965
Enzymic digestion, acidic hydrolysis, deuterium exchange, infrared absorption spectra, and differential thermal analysis of chitins show that not only do the structures of <x- and ~-chitins differ…
Muramidase Catalyzed Hydrolysis of Glycol Chitin
- Biology
- 1964
The mode of action of muramidase on glycol chitin was investigated following the degradation of glycol Chitin by the estimation of reducing power produced by hydrolysis and the optimum temperature as well as pH were found to lie at 50°C and pH 5 respectively, which nearly agree with those obtained by α viscosimetric determination of mur amidase activity.
Chitinase of Streptomyces sp: purification and properties.
- BiologyEnzymologia
- 1970
Streptomyces sp chitinase purification and properties by column chromatography, noting calcium component
Structure of Some Crystalline Lysozyme-Inhibitor Complexes Determined by X-Ray Analysis At 6 Å Resolution
- ChemistryNature
- 1965
Structure of Some Crystalline Lysozyme-Inhibitor Complexes Determined by X-Ray Analysis At 6 A Resolution