Adsorption and reactions of chitinase and lysozyme on chitin

@article{Skujins2005AdsorptionAR,
  title={Adsorption and reactions of chitinase and lysozyme on chitin},
  author={John Skujins and A. H. Pukite and A. D. Mclaren},
  journal={Molecular and Cellular Biochemistry},
  year={2005},
  volume={2},
  pages={221-228}
}
SummaryIsotherms for adsorption of chitinase on chitin and lysozyme on chitin have been determined at two temperatures and rates of hydrolysis of chitin catalysed by these enzymes have been measured at three temperatures and at several enzyme concentrations for each. Ribonuclease, not an enzyme for chitin, and heat-denatured lysozyme and chitinase show reduced or no adsorption to this substrate.Initial hydrolysis rates of chitin by both enzymes are proportional to total enzyme concentrations in… 
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Bacterial chitin degradation—mechanisms and ecophysiological strategies
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This review summarizes different mechanisms and the principal steps involved in chitin degradation at a molecular level while also discussing the coupling of community composition to measured chitIn hydrolysis activities and substrate uptake.
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An efficient enzymatic production of N-acetyl-d-glucosamine from crude chitin powders
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An efficient affinity adsorption–enzymatic reaction integrated approach to produce N-acetyl-D-glucosamine (GlcNAc) from crude chitin powders (CP) with high purity with easy separation from hydrolysates with a high purity of 98%.
Chitin-based magnetic composite for the removal of contaminating substances from aqueous media
A method of obtaining multipurpose magnetic chitin, which combines the magnetic properties of magnetite and the adsorption properties of polysaccharide, was proposed. The possibility of using…
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Four extracellular proteins with chitinase activity capable of binding chitin substrates have been revealed in the culture liquid of chitinase superproducing mutant strain M-1 of Serratia marcescens.…
Fungal mycelium--the source of chitosan for chromatography.
  • J. Kucera
  • Biology
    Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
  • 2004
Effect of Chitin and Chitooligosaccharide on In vitro Growth of Lactobacillus rhamnosus GG and Escherichia coli TG
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Chitooligosaccharides appear promising as potential prebiotic compounds associated with insect food products and should be verified in well-designed human studies.
Heterologous expression and characterization of an antifungal chitinase (Chit46) from Trichoderma harzianum GIM 3.442 and its application in colloidal chitin conversion.
Cadaverine Production From L-Lysine With Chitin-Binding Protein-Mediated Lysine Decarboxylase Immobilization
TLDR
Results demonstrate that immobilization of CadA using chitin-binding domain has the potential in cadaverine production on an industrial scale.
Isolation and characterization of extracellular lipase preparations from wild-type (V-10) and mutant (M-1)Serratia marcescens strains
TLDR
The cultivation conditions of wild-type strain V-10 and mutant strain M-l (overproducer of endonuclease and chitinase) ofSerratia marcescens optimal for extracellular lipase biosynthesis were determined and it was shown that extracllular lipases from V- 10 and M-1 can be precipitated in a weakly acidic medium.
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References

SHOWING 1-10 OF 19 REFERENCES
THE HYDROLYSIS OF CHITIN BY CONCENTRATED HYDROCHLORIC ACID, AND THE PREPARATION OF LOW-MOLECULAR-WEIGHT SUBSTRATES FOR LYSOZYME.
  • J. A. Rupley
  • Chemistry, Biology
    Biochimica et biophysica acta
  • 1964
Studies on Chitin
TLDR
There is only a weak bonding between the chitin and the water-soluble cuticular protein, and an increase to pH 9 is necessary before all the adsorbed protein can be removed.
Studies on chitin. VI. The nature of alpha- and beta-chitins.
Enzymic digestion, acidic hydrolysis, deuterium exchange, infrared absorption spectra, and differential thermal analysis of chitins show that not only do the structures of <x- and ~-chitins differ…
Muramidase Catalyzed Hydrolysis of Glycol Chitin
TLDR
The mode of action of muramidase on glycol chitin was investigated following the degradation of glycol Chitin by the estimation of reducing power produced by hydrolysis and the optimum temperature as well as pH were found to lie at 50°C and pH 5 respectively, which nearly agree with those obtained by α viscosimetric determination of mur amidase activity.
Ribonuclease adsorption on glass surfaces.
The Chitin/Protein Complexes of Insect Cuticles
Application of scanning and transmitting electron micrography in the differentiation of soil streptomycetes
Chitinase of Streptomyces sp: purification and properties.
Streptomyces sp chitinase purification and properties by column chromatography, noting calcium component
Structure of Some Crystalline Lysozyme-Inhibitor Complexes Determined by X-Ray Analysis At 6 Å Resolution
Structure of Some Crystalline Lysozyme-Inhibitor Complexes Determined by X-Ray Analysis At 6 A Resolution
The Adsorption and Reactions of Enzymes and Proteins on Kaolinite. I
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