Adrenergic neurons in human brain demonstrated by immunohistochemistry with antibodies to phenylethanolamine-N-methyltransferase (PNMT): Discovery of a new group in the nucleus tractus solitarius

  title={Adrenergic neurons in human brain demonstrated by immunohistochemistry with antibodies to phenylethanolamine-N-methyltransferase (PNMT): Discovery of a new group in the nucleus tractus solitarius},
  author={Kunio Kitahama and John Pearson and Luc Denoroy and N. Rodr{\'i}guez de Kopp and J Ulrich and T. Maeda and Michel Jouvet},
  journal={Neuroscience Letters},
Adrenaline neurons in human brain were demonstrated by immunohistochemistry using antibody to phenylethanolamine-N-methyltransferase (PNMT), the final enzyme in the pathway of adrenaline synthesis, using fixed frozen sections and a highly sensitive free floating technique which utilizes metallization. Small densely packed PNMT-immunoreactive neurons were observed to form a nucleus lying just ventrolateral to the area postrema and in the dorsal part of the nucleus tractus solitarius. Larger… Expand
Adrenergic neurons in sheep brain demonstrated by immunohistochemistry with antibodies to phenylethanolamine N-methyltransferase (PNMT) and dopamine-β-hydroxylase (DBH): Absence of the C1 cell group in the sheep brain
  • Y. Tillet
  • Biology, Medicine
  • Neuroscience Letters
  • 1988
Compared with rodents or primates, this animal presents a different pattern of central adrenergic innervation and could be an alternative model to study the central role of adrenaline in various physiological functions as different as swallowing or reproduction. Expand
Distribution of tyrosine hydroxylase and neuropeptide Y–like immunoreactive neurons in rabbit medulla oblongata, with attention to colocalization studies, presumptive adrenaline‐synthesizing perikarya, and vagal preganglionic cells
The distribution of tyrosine hydroxylase–containing neurons in the caudal medulla was in accordance with previous descriptions of the A1 and A2 groups based on the unenhanced FAGLU procedure. Expand
Distribution of phenylethanolamine N‐methyltransferase cell bodies, axons, and terminals in monkey brainstem: An immunohistochemical mapping study
A comparison with maps of other catecholamine neurons in primates is discussed, confirming that the distribution of the adrenergic system in monkeys is similar to that described in the human. Expand
Presumptive adrenergic neurons containing phenylethanolamine N-methyltransferase immunoreactivity in the medulla oblongata of neonatal swine
The general regional organization of neurons expressing PNMT (-like) immunoreactivity (ir) in the neonatal swine was similar to data obtained in other species and, in some aspects, more closely resembled the pattern observed in the primate brain. Expand
Immunohistochemistry of tyrosine hydroxylase and phenylethanolamine N-methyltransferase in the human brain stem: Description of adrenergic perikarya and characterization of longitudinal catecholaminergic pathways
Using immunocytochemical method in conjunction with antibodies to tyrosine hydroxylase and phenylethanolamine N-methyltransferase, catecholaminergic cell groups and axon pathways are mapped in theExpand
Co-localization of tyrosine hydroxylase and phenylethanolamine-N-methyltransferase immunoreactivity in the rat retina: a re-examination using double labeling on semi-thin sections.
The result of these experiments clearly indicate that the populations of TH-positive cells and PNMT-positive (presumably epinephrinergic) cells are separated, and raises the question of the enzymatic activity of PN MT, which appears to be different from the classical pathway of catecholamine biosynthesis in the retina. Expand
Absence of adrenaline neurons in the guinea pig brain: A combined immunohistochemical and high-performance liquid chromatography study
Guinea pigs lack central neurons capable of synthesizing adrenaline, and the central nervous systems of both normal and colchicine-treated guinea pigs failed to demonstrate immunoreactivity for PNMT, the synthetic enzyme for adrenaline. Expand
Differential organization of synapses immunoreactive to phenylethanolamine-N-methyltransferase or neuropeptide Y in the parvicellular compartments of the hypothalamic paraventricular nucleus of the rat
  • G. Alonso
  • Medicine, Biology
  • Journal of Chemical Neuroanatomy
  • 1993
Data indicate that the large majority of NPY-immunoreactive fibres detected within the pPVN arise from non-catecholaminergic neurons located in the mediobasal hypothalamus and mainly form symmetric synapses on neurons of the pVN, whereas only a minority of them arise from hindbrain regions, and like PNMT fibres innervating this nucleus preferentially form asymmetric axo-dendritic synapses. Expand
Descending phenylethanolamine-N-methyltransferase projections to the monkey spinal cord: an immunohistochemical double labeling study
It is determined that a population of spinally projecting neurons in the monkey brainstem also contained the enzyme phenylethanolamine-N-methyltransferase (PNMT), and possible functional roles of descending PNMT cells include involvement in sympathetic control of cardiovascular mechanisms and/or tonic descending inhibition of dorsal horn neurons. Expand
Morphological Characteristics of C1 and C2 Adrenergic Neurone Groups in Marmoset Monkey Brainstem by using Antibody against Phenylethanolamine‐N‐methyltransferase
The results provide information upon the adrenergic system in the medulla oblongata of a species that presents a useful model of a small primate brain, the marmoset monkey. Expand


Immunohistochemical evidence for the existence of adrenaline neurons in the rat brain
The hypothesis is given that the PNMT containing neurons represent A containing neurons, and that A may act as a neurotransmitter in the rat brain. Expand
Simultaneous demonstration of phenylethanolamine N-methyltransferase immunofluorescent and catecholamine fluorescent nerve cell bodies in the rat medulla oblongata
Inhibition of phenylethanolamine N -methyltransferase and monoamine oxidase results in the appearance of catecholamine fluorescence in the immunoreactive cell bodies suggesting that they usually store adrenaline which reacts poorly with the formaldehyde/glutaraldehyde mixture or other aldehydes which induce catecholinamines fluorescence. Expand
Distribution of adrenaline-synthesizing enzyme activity in the human brain
The distribution of PNMT activity in normal human brain was found to be similar to that in the rat, but, in addition, important activity was found in the substantia nigra, internal pallidium and nucleus accumbens. Expand
Differential co-existence of neuropeptide Y (NPY)-like immunoreactivity with catecholamines in the central nervous system of the rat
The findings indicate that central catecholamine neurons can be subdivided into distinct sub-groups based upon the coexistence of a specific peptide. Expand
Localisation of phenylethanolamine N-methyl transferase in the rat brain nuclei
The findings suggest that adrenaline may serve as a neurotransmitter in the central nervous system, and the distribution of the enzyme in specific nuclei of the rat brain is studied. Expand
Human brainstem catecholamine neuronal anatomy as indicated by immunocytochemistry with antibodies to tyrosine hydroxylase
Broad agreement of results increases the confidence with which tyrosine hydroxylase-like immunoreactivity can be used to trace catecholaminergic pathways in human postmortem material. Expand
An immunohistochemical study of the organization of catecholaminergic cells and terminal fields in the paraventricular and supraoptic nuclei of the hypothalamus
It is concluded that adrenergic inputs to the paraventricular nucleus may influence cells that project to the median eminence and to preganglionic autonomic cell groups in the medulla and spinal cord. Expand
Immunohistochemical study of the catecholaminergic systems in the lower brain stem of the human infant.
This technique might usefully be applied to the exploration of putative diseases of bulbopontine CA systems, such as the sudden infant death syndrome and central blood pressure disorders. Expand
Distribution of dopamine‐, noradrenaline‐, and adrenaline‐containing cell bodies in the rat medulla oblongata: Demonstrated by the immunocytochemical localization of catecholamine biosynthetic enzymes
The detection of perikarya which show immunoreactivity for TH, used in the biosynthesis of dopamine, noradrenaline, and adrenaline, but not DBH, which converts dopamine to norad Renaline, suggests the existence of dopamine‐synthesiz‐ing neurons in the medulla. Expand
Localization and characterization of phenylethanolamine N-methyl transferase in the brain of various mammalian species
Immunotitration studies revealed cross-reactivity between the homologous adrenal and brain PNMT, which has the same substrate specificity and similar kinetic properties as the adrenal enzyme. Expand