Adjuvant and immunostimulatory effects of LPS and β-glucan on immune response in Japanese flounder, Paralichthys olivaceus.

@article{Diao2013AdjuvantAI,
  title={Adjuvant and immunostimulatory effects of LPS and $\beta$-glucan on immune response in Japanese flounder, Paralichthys olivaceus.},
  author={J. Diao and H. Ye and Xiao-Qing Yu and Ying Fan and La Xu and Tianbao Li and Yong-qiang Wang},
  journal={Veterinary immunology and immunopathology},
  year={2013},
  volume={156 3-4},
  pages={
          167-75
        }
}
In order to investigate the adjuvant and immunostimulatory effects of LPS and β-glucan, a highly effective vaccine candidate for Edwardsiella tarda known as glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was recombinantly expressed in Escherichia coli, which was intramuscularly injected into Japanese flounder alone or containing LPS or β-glucan. ELISA analysis of sera showed that the fish immunized with rGAPDH containing LPS or β-glucan had significant higher antibody titers than rGAPDH… Expand
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