Adjustments on the cryopreservation conditions reduce the incidence of boar ejaculates with poor sperm freezability.

@article{Hernndez2007AdjustmentsOT,
  title={Adjustments on the cryopreservation conditions reduce the incidence of boar ejaculates with poor sperm freezability.},
  author={M Isabel Hern{\'a}ndez and Jordi Roca and Maria A Gil and Juan Mar{\'i}a V{\'a}zquez and Emilio Martinez},
  journal={Theriogenology},
  year={2007},
  volume={67 9},
  pages={1436-45}
}
The objective of the present study was to evaluate the effectiveness of different cryopreservation conditions (CCs) for freezing and thawing boar ejaculates, focusing on those having sub-optimal sperm freezability. Using a split-ejaculate technique, single ejaculates from 53 boars were diluted in lactose-egg yolk extender, containing a final glycerol concentration (GLY) of 2 or 3%, packaged in 0.5 mL straws and were cooled at rates of -10, -40 or -60 degrees C/min (cooling rate: CR). Thereafter… CONTINUE READING

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