Adjustment of Cell-Type Composition Minimizes Systematic Bias in Blood DNA Methylation Profiles Derived by DNA Collection Protocols

@inproceedings{Shiwa2016AdjustmentOC,
  title={Adjustment of Cell-Type Composition Minimizes Systematic Bias in Blood DNA Methylation Profiles Derived by DNA Collection Protocols},
  author={Yuh Shiwa and Tsuyoshi Hachiya and Ryohei Furukawa and Hideki Ohmomo and Kanako Ono and Hisaaki Kudo and Jun Hata and Atsushi Hozawa and Motoki Iwasaki and Koichi Matsuda and Naoko Minegishi and Mamoru Satoh and Kozo Tanno and Taiki Yamaji and Kenji Wakai and Jiro Hitomi and Yutaka Kiyohara and Michiaki Kubo and Hideo Tanaka and Shoichiro Tsugane and Masayuki Yamamoto and Kenji Sobue and Atsushi Shimizu and Devin C. Koestler},
  booktitle={PloS one},
  year={2016}
}
Differences in DNA collection protocols may be a potential confounder in epigenome-wide association studies (EWAS) using a large number of blood specimens from multiple biobanks and/or cohorts. Here we show that pre-analytical procedures involved in DNA collection can induce systematic bias in the DNA methylation profiles of blood cells that can be adjusted by cell-type composition variables. In Experiment 1, whole blood from 16 volunteers was collected to examine the effect of a 24 h storage… CONTINUE READING
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