Adjustable mutations in lactate (LA)-polymerizing enzyme for the microbial production of LA-based polyesters with tailor-made monomer composition.

@article{Yamada2010AdjustableMI,
  title={Adjustable mutations in lactate (LA)-polymerizing enzyme for the microbial production of LA-based polyesters with tailor-made monomer composition.},
  author={Miwa Yamada and Ken'ichiro Matsumoto and Kotaro Shimizu and Shu Uramoto and Takanori Nakai and Fumi Shozui and Seiichi Taguchi},
  journal={Biomacromolecules},
  year={2010},
  volume={11 3},
  pages={815-9}
}
Lactate (LA)-polymerizing enzyme (LPE) is a newly established class of polyhydroxyalkanoate (PHA) synthase, which can incorporate LA units into a polymer chain. We previously synthesized P(LA-co-3-hydroxybutyrate)s [P(LA-co-3HB)s] in recombinant Escherichia coli using the first LPE, which is the Ser325Thr/Glu481Lys mutant of PHA synthase from Pseudomonas sp. 61-3 [PhaC1(Ps)ST/QK]. In this study, we finely regulated LA fraction in the copolymer by saturated mutations at position 392 (F392X… CONTINUE READING

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