Adherence to and cytotoxicity of Escherichia coli for eucaryotic cell lines quantified by MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide).

@article{Kreft1992AdherenceTA,
  title={Adherence to and cytotoxicity of Escherichia coli for eucaryotic cell lines quantified by MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide).},
  author={Burkhard Kreft and Ole Carstensen and Eberhard Straube and Sabine Bohnet and Joerg Hacker and Reinhard Marre},
  journal={Zentralblatt fur Bakteriologie : international journal of medical microbiology},
  year={1992},
  volume={276 2},
  pages={231-42}
}
Adherence of Escherichia coli to human epithelial cells (HEp-2) was studied using MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) which is cleaved by enzymes of eucaryotic or procaryotic cells to formazan. This method allows to quantify adherence of Escherichia coli to HEp-2 cells and offers the advantage of assaying a large number of… CONTINUE READING