Adenylate energy charge in Saccharomyces cerevisiae during starvation

  title={Adenylate energy charge in Saccharomyces cerevisiae during starvation},
  author={William J. Ball and Daniel E. Atkinson},
  journal={Journal of Bacteriology},
  pages={975 - 982}
Bakers' yeast cells, Saccharomyces cerevisiae, if grown aerobically on ethanol or if grown aerobically on glucose and allowed to pass into stationary phase, with utilization of accumulated ethanol, maintain a normal value (0.8 to 0.9) of the adenylate energy charge during prolonged starvation. In contrast, cells grown anaerobically on glucose and cells in the early stages of aerobic growth on glucose exhibit a rapid decrease of energy charge if transferred to medium lacking on energy source… 

Functional capacities and the adenylate energy charge in Escherichia coli under conditions of nutritional stress

Functional capacities in Escherichia coli cells starved for glucose were examined by comparing protein synthesis, utilization of new substrates, and maintenance of viability with the adenylate energy


The kinetics and extent of glucose uptake, ethanol and acetate production, glucose-induced medium acidification and endogenous respiration were studied in baker's yeast starved aerobically for 0, 28,


The results revealed that the initial pH value of the medium affected the pattern of glycogen and trehalose degradation and consequently, also caused the loss of viability and cell lysis in aerobically starving baker's yeast.

Changes in intermediate levels during batch culture of Saccharomyces cerevisiae

Bakers' yeast has been grown on a medium containing 1% glucose in aerobic conditions and the levels of ATP, AMP, glucose 6-phosphate, fructose 1,6- diph phosphate, 6- phosphogluconate, citrate and glyoxylate showed differences in the different phases of the fermentation and can be used as indicators of metabolic state.

Anaerobic homolactate fermentation with Saccharomyces cerevisiae results in depletion of ATP and impaired metabolic activity.

The anticipated increase in maximal velocity (V(max) of glycolytic enzymes was not observed in homolactate fermentation suggesting the absence of protein synthesis that may be attributed to decreased energy availability, which hinders protein synthesis, central carbon metabolism and subsequent energy generation.

Investigation of adenylate energy charge, phosphorylation potential, and ATP concentration in cells stressed with starvation and heat

Cells were, however, sensitized to heat under nutritionally deprived conditions, a finding which may be significant for tumor treatment by heat in vivo.

Oxygen availability strongly affects chronological lifespan and thermotolerance in batch cultures of Saccharomyces cerevisiae

It is indicated that the fast transition from glucose excess to SP in anaerobic cultures precludes acquisition of longevity and thermotolerance, and demonstrates the importance of a preceding, calorie-restricted conditioning phase in the acquisition ofongevity and stress tolerance in SP yeast cultures, irrespective of oxygen availability.

Intracellular accumulation of AMP as a cause for the decline in rate of ethanol production by Saccharomyces cerevisiae during batch fermentation

The coupling of glycolytic flux and fermentation to cell growth via degradation products of RNA may be of evolutionary advantage for Saccharomyces cerevisiae and would reduce the exposure of nongrowing cells to potentially harmful concentrations of waste products from metabolism and would conserve nutrients for future growth under more favorable conditions.

Adenylate energy charge during batch culture of Beneckea natriegens.

The value of the adenylate energy charge during batch culture of Beneckea natriegens remained relatively constant during the exponential and early stationary phases of the growth cycle, and on cessation of growth significant variation in bacterial ATP content was observed.

Relationship of Glycolytic Intermediates, Glycolytic Enzymes, and Ammonia to Glycogen Metabolism During Sporulation in the Yeast Saccharomyces cerevisiae

Most of the changes in the glycolytic pathway that occur during sporulation, with the exception of glycogen degradation and the concomitant increase in intracellular glucose pools, are a response to the transfer to sporulation medium and are independent of sporulation-specific processes.



Adenylate Energy Charge in Escherichia coli During Growth and Starvation

Tabulation of adenylate concentrations previously reported for various organisms and tissues supports the prediction, based on enzyme kinetic observations in vitro, that the energy charge is stabilized near 0.85 in intact metabolizing cells of a wide variety of types.

Glucose and Fructose Metabolism in a Phosphoglucoisomeraseless Mutant of Saccharomyces cerevisiae

The kinetics of glucose-6-phosphate accumulation during glucose utilization in the mutant is not consistent with the notion that the utilization of glucose is controlled by glucose- 6-ph phosphate, which suggests a C-l preferential cleavage.

Changes in the intracellular concentrations of adenosine phosphates and nicotinamide nucleotides during the aerobic growth cycle of yeast on different carbon sources.

The adenylate-kinase reaction was found to be far from equilibrium in the glucose-grown cells and when glucose was still present in the growth medium, and ADP is suggested to be the inducer of formation of respiratory enzymes.

Adenosine phosphates and the control of glycolysis and gluconeogenesis in yeast.

It is concluded that adenosine phosphates do not control the direction of glycolytic flux in yeast and an alternative control of gly colysis and gluconeogenesis by enzyme activation and inactivation is suggested.

The behaviour of nucleic acids in synchronously dividing cultures of Saccharomyces cerevisiae.

Biochemical studies of bacterial sporulation and germination. XXII. Energy metabolism in early stages of germination of Bacillus megaterium spores.

Abstract Dormant spores of Bacillus megaterium contain DPN+, TPN+, and FAD at levels comparable to those in log phase vegetative cells, but little or no reduced pyridine nucleotides are present. The


Fluctuating metabolite levels in yeast cells and extracts, and the control of phosphofructokinase activity in vitro.