Adenovirus serotype 7 retention in a late endosomal compartment prior to cytosol escape is modulated by fiber protein.

@article{Miyazawa2001AdenovirusS7,
  title={Adenovirus serotype 7 retention in a late endosomal compartment prior to cytosol escape is modulated by fiber protein.},
  author={Naoki Miyazawa and Ronald G. Crystal and Philip L. Leopold},
  journal={Journal of virology},
  year={2001},
  volume={75 3},
  pages={1387-400}
}
The intracellular trafficking of adenovirus (Ad) subgroup B (e.g., Ad7) differs from that of subgroup C (e.g., Ad5) in that Ad5 rapidly escapes from endocytic compartments following infection whereas Ad7 accumulates in organelles. To assess the hypothesis that Ad7 is targeted to the lysosomal pathway, Ad7 and Ad5 were conjugated with fluorophores and their trafficking in A549 epithelial cells was analyzed by fluorescence microscopy. Within 1 h after infection, Ad7, but not Ad5, accumulated in… CONTINUE READING